Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30714 | 3' | -52.3 | NC_006552.1 | + | 26287 | 0.66 | 0.880963 |
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Target: 5'- uAGGAGCGGcGCaCCGgGAu---ACCGUc -3' miRNA: 3'- cUCCUUGUC-CG-GGCgCUuuuuUGGCA- -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 18672 | 0.66 | 0.873142 |
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Target: 5'- cAGGGACuucaGCCCGCGccu-GGCCGa -3' miRNA: 3'- cUCCUUGuc--CGGGCGCuuuuUUGGCa -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 4755 | 0.68 | 0.802013 |
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Target: 5'- -uGGAcCAGGCCCGCcaggcacgcaagGAAAAGcacacccacACCGUg -3' miRNA: 3'- cuCCUuGUCCGGGCG------------CUUUUU---------UGGCA- -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 17649 | 0.68 | 0.782196 |
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Target: 5'- --aGAACAGGCCacaGCGAGccAAGGCCu- -3' miRNA: 3'- cucCUUGUCCGGg--CGCUU--UUUUGGca -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 30877 | 0.7 | 0.686457 |
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Target: 5'- uGAGGGuguuggaccACAGGCCCGCaucGAcgcgGAAGAUCGg -3' miRNA: 3'- -CUCCU---------UGUCCGGGCG---CU----UUUUUGGCa -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 17255 | 0.74 | 0.468492 |
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Target: 5'- aGAGcuGCAGGCCCGCGccugcaagguGAAAGCCGc -3' miRNA: 3'- -CUCcuUGUCCGGGCGCu---------UUUUUGGCa -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 62415 | 0.74 | 0.448332 |
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Target: 5'- cGAGG-GCGGG-CCGCGGAAAcAGCCGUu -3' miRNA: 3'- -CUCCuUGUCCgGGCGCUUUU-UUGGCA- -5' |
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| 30714 | 3' | -52.3 | NC_006552.1 | + | 9428 | 1.08 | 0.002518 |
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Target: 5'- cGAGGAACAGGCCCGCGAAAAAACCGUg -3' miRNA: 3'- -CUCCUUGUCCGGGCGCUUUUUUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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