Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30715 | 5' | -57.8 | NC_006552.1 | + | 55711 | 1.05 | 0.001063 |
Target: 5'- gAGACGCGCACCCCUGCUGAAAGCCUUc -3' miRNA: 3'- -UCUGCGCGUGGGGACGACUUUCGGAA- -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 32647 | 0.76 | 0.141346 |
Target: 5'- cGA-GCGCGCUCCUGCUGGgccugcuGAGCCUg -3' miRNA: 3'- uCUgCGCGUGGGGACGACU-------UUCGGAa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 20105 | 0.68 | 0.440732 |
Target: 5'- cGGCGCGCAcggaugcCCCCUGCaucagcGCCUUg -3' miRNA: 3'- uCUGCGCGU-------GGGGACGacuuu-CGGAA- -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 53997 | 0.68 | 0.451439 |
Target: 5'- cAGGCGCGUGgCCCUGgccCUGGccgcGAGCCg- -3' miRNA: 3'- -UCUGCGCGUgGGGAC---GACU----UUCGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 36063 | 0.68 | 0.461294 |
Target: 5'- cAGGCGUcUugCCCUGUUGGucGCCa- -3' miRNA: 3'- -UCUGCGcGugGGGACGACUuuCGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 17677 | 0.67 | 0.501794 |
Target: 5'- aGGAaGCGCGCgCCCUGCcccauuuccUGuAGAGCCg- -3' miRNA: 3'- -UCUgCGCGUG-GGGACG---------AC-UUUCGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 7213 | 0.67 | 0.501794 |
Target: 5'- cGGCGCGCagcgaGCCgCUGCcGcgGGCCUg -3' miRNA: 3'- uCUGCGCG-----UGGgGACGaCuuUCGGAa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 8532 | 0.66 | 0.543777 |
Target: 5'- cGACGCGCACCggCUUGCUGuccacGUCg- -3' miRNA: 3'- uCUGCGCGUGG--GGACGACuuu--CGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 56627 | 0.66 | 0.543777 |
Target: 5'- cGGCGCGgcuuCugUCCUGCUgcgGAAGGCCc- -3' miRNA: 3'- uCUGCGC----GugGGGACGA---CUUUCGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 25704 | 0.66 | 0.55446 |
Target: 5'- -cAUGcCGCACUCCUGCgcagcgauUGAAGGCCc- -3' miRNA: 3'- ucUGC-GCGUGGGGACG--------ACUUUCGGaa -5' |
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30715 | 5' | -57.8 | NC_006552.1 | + | 43933 | 0.66 | 0.565203 |
Target: 5'- -cGCGCGCACUuuUGCgcc-AGCCUUa -3' miRNA: 3'- ucUGCGCGUGGggACGacuuUCGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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