Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30716 | 3' | -61.5 | NC_006552.1 | + | 17667 | 0.66 | 0.40988 |
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Target: 5'- gUUGGCg--GCGaggaaGCGcGCGCCCUGCc- -3' miRNA: 3'- aGACCGacaCGC-----UGC-CGCGGGACGag -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 50416 | 0.66 | 0.383621 |
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Target: 5'- aUCUGGgUGgGCGGCcugauuGGCGgccuccucuUCCUGCUCa -3' miRNA: 3'- -AGACCgACaCGCUG------CCGC---------GGGACGAG- -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 15484 | 0.68 | 0.326841 |
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Target: 5'- --cGGUUGUGCGGCuuGCGCC-UGCUg -3' miRNA: 3'- agaCCGACACGCUGc-CGCGGgACGAg -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 28665 | 0.68 | 0.326841 |
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Target: 5'- -aUGGCUGcaguggugaucUGCGGCGGCGCCaCgccGCc- -3' miRNA: 3'- agACCGAC-----------ACGCUGCCGCGG-Ga--CGag -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 12309 | 0.7 | 0.244523 |
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Target: 5'- cCUGaGCcaugGUGCGuagucgcucgGCGGCGCCCUGaCUg -3' miRNA: 3'- aGAC-CGa---CACGC----------UGCCGCGGGAC-GAg -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 42863 | 0.7 | 0.238502 |
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Target: 5'- gCUGGCUGUGCaguucccgaACGGCGUCgaGCa- -3' miRNA: 3'- aGACCGACACGc--------UGCCGCGGgaCGag -5' |
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| 30716 | 3' | -61.5 | NC_006552.1 | + | 54733 | 1.08 | 0.00031 |
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Target: 5'- uUCUGGCUGUGCGACGGCGCCCUGCUCc -3' miRNA: 3'- -AGACCGACACGCUGCCGCGGGACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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