miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30716 3' -61.5 NC_006552.1 + 17667 0.66 0.40988
Target:  5'- gUUGGCg--GCGaggaaGCGcGCGCCCUGCc- -3'
miRNA:   3'- aGACCGacaCGC-----UGC-CGCGGGACGag -5'
30716 3' -61.5 NC_006552.1 + 50416 0.66 0.383621
Target:  5'- aUCUGGgUGgGCGGCcugauuGGCGgccuccucuUCCUGCUCa -3'
miRNA:   3'- -AGACCgACaCGCUG------CCGC---------GGGACGAG- -5'
30716 3' -61.5 NC_006552.1 + 15484 0.68 0.326841
Target:  5'- --cGGUUGUGCGGCuuGCGCC-UGCUg -3'
miRNA:   3'- agaCCGACACGCUGc-CGCGGgACGAg -5'
30716 3' -61.5 NC_006552.1 + 28665 0.68 0.326841
Target:  5'- -aUGGCUGcaguggugaucUGCGGCGGCGCCaCgccGCc- -3'
miRNA:   3'- agACCGAC-----------ACGCUGCCGCGG-Ga--CGag -5'
30716 3' -61.5 NC_006552.1 + 12309 0.7 0.244523
Target:  5'- cCUGaGCcaugGUGCGuagucgcucgGCGGCGCCCUGaCUg -3'
miRNA:   3'- aGAC-CGa---CACGC----------UGCCGCGGGAC-GAg -5'
30716 3' -61.5 NC_006552.1 + 42863 0.7 0.238502
Target:  5'- gCUGGCUGUGCaguucccgaACGGCGUCgaGCa- -3'
miRNA:   3'- aGACCGACACGc--------UGCCGCGGgaCGag -5'
30716 3' -61.5 NC_006552.1 + 54733 1.08 0.00031
Target:  5'- uUCUGGCUGUGCGACGGCGCCCUGCUCc -3'
miRNA:   3'- -AGACCGACACGCUGCCGCGGGACGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.