Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30718 | 5' | -53.4 | NC_006552.1 | + | 33395 | 0.66 | 0.88168 |
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Target: 5'- aGGGCgGCggcgcuuagACuGCGCCGAGGcgcucgccGUGGa -3' miRNA: 3'- -CCUGgUGa--------UGuCGUGGCUCCau------CACC- -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 31399 | 0.66 | 0.88168 |
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Target: 5'- -cACCGucgGCGGCGCCGccaGUGGUGGu -3' miRNA: 3'- ccUGGUga-UGUCGUGGCuc-CAUCACC- -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 26519 | 0.67 | 0.81428 |
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Target: 5'- -uGCCACcagcagcgGCGGCGCCGGGGUGu--- -3' miRNA: 3'- ccUGGUGa-------UGUCGUGGCUCCAUcacc -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 33647 | 0.67 | 0.81428 |
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Target: 5'- cGG-UCACgcCGGCACCGAaGUGGUcGGa -3' miRNA: 3'- -CCuGGUGauGUCGUGGCUcCAUCA-CC- -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 14432 | 0.67 | 0.795371 |
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Target: 5'- --cCCGCUGCuGGCAUC--GGUGGUGGa -3' miRNA: 3'- ccuGGUGAUG-UCGUGGcuCCAUCACC- -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 18360 | 0.68 | 0.775791 |
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Target: 5'- uGACCACga-AGCGCCGGcGG-AGUGu -3' miRNA: 3'- cCUGGUGaugUCGUGGCU-CCaUCACc -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 2663 | 0.75 | 0.37515 |
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Target: 5'- gGGACCGC-GCGGCGCCGAucaacgcucgGGUcgagaaAGUGGc -3' miRNA: 3'- -CCUGGUGaUGUCGUGGCU----------CCA------UCACC- -5' |
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| 30718 | 5' | -53.4 | NC_006552.1 | + | 53917 | 1.13 | 0.00121 |
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Target: 5'- aGGACCACUACAGCACCGAGGUAGUGGu -3' miRNA: 3'- -CCUGGUGAUGUCGUGGCUCCAUCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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