miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30719 3' -57.3 NC_006552.1 + 20951 0.66 0.644884
Target:  5'- uCCGGGcguccGGCACCGGGauggccgUCaUGCUCg-- -3'
miRNA:   3'- -GGCUCa----CCGUGGCCC-------AGcACGAGaaa -5'
30719 3' -57.3 NC_006552.1 + 24717 0.66 0.650254
Target:  5'- uUCGGGUcguaggccaccagccGGCGCCGGGcC-UGCUCg-- -3'
miRNA:   3'- -GGCUCA---------------CCGUGGCCCaGcACGAGaaa -5'
30719 3' -57.3 NC_006552.1 + 28813 0.68 0.554073
Target:  5'- aCCaGGGUGGCGgguucaacucgcuguCCGGGUCGUaCUCc-- -3'
miRNA:   3'- -GG-CUCACCGU---------------GGCCCAGCAcGAGaaa -5'
30719 3' -57.3 NC_006552.1 + 53791 1.08 0.000934
Target:  5'- aCCGAGUGGCACCGGGUCGUGCUCUUUg -3'
miRNA:   3'- -GGCUCACCGUGGCCCAGCACGAGAAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.