Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30719 | 3' | -57.3 | NC_006552.1 | + | 20951 | 0.66 | 0.644884 |
Target: 5'- uCCGGGcguccGGCACCGGGauggccgUCaUGCUCg-- -3' miRNA: 3'- -GGCUCa----CCGUGGCCC-------AGcACGAGaaa -5' |
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30719 | 3' | -57.3 | NC_006552.1 | + | 24717 | 0.66 | 0.650254 |
Target: 5'- uUCGGGUcguaggccaccagccGGCGCCGGGcC-UGCUCg-- -3' miRNA: 3'- -GGCUCA---------------CCGUGGCCCaGcACGAGaaa -5' |
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30719 | 3' | -57.3 | NC_006552.1 | + | 28813 | 0.68 | 0.554073 |
Target: 5'- aCCaGGGUGGCGgguucaacucgcuguCCGGGUCGUaCUCc-- -3' miRNA: 3'- -GG-CUCACCGU---------------GGCCCAGCAcGAGaaa -5' |
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30719 | 3' | -57.3 | NC_006552.1 | + | 53791 | 1.08 | 0.000934 |
Target: 5'- aCCGAGUGGCACCGGGUCGUGCUCUUUg -3' miRNA: 3'- -GGCUCACCGUGGCCCAGCACGAGAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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