Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30722 | 5' | -45 | NC_006552.1 | + | 62073 | 0.66 | 0.999128 |
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Target: 5'- --gGGUUCGAcauccugguGGugcAUGAGGAUGGCCg -3' miRNA: 3'- gugUCAAGUU---------CCuu-UGCUCUUACUGG- -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 47619 | 0.67 | 0.998842 |
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Target: 5'- uGCAGcUUCGAGcaccgcAAGCGAGAgucggcagcaaagcGUGGCCg -3' miRNA: 3'- gUGUC-AAGUUCc-----UUUGCUCU--------------UACUGG- -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 3741 | 0.7 | 0.98563 |
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Target: 5'- gGguGUUCGcGGggGCGAcaucAAUGGCCg -3' miRNA: 3'- gUguCAAGUuCCuuUGCUc---UUACUGG- -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 49539 | 0.71 | 0.976185 |
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Target: 5'- aCAcCAGUUCAGcGAggUGGGGAUGAUg -3' miRNA: 3'- -GU-GUCAAGUUcCUuuGCUCUUACUGg -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 35128 | 0.73 | 0.939786 |
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Target: 5'- gGCGGUUCAGGGu--CGAGuugcGGCCc -3' miRNA: 3'- gUGUCAAGUUCCuuuGCUCuua-CUGG- -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 46757 | 0.75 | 0.862306 |
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Target: 5'- gCACAGUccUCcGGGAGuCGGGAGUaGACCc -3' miRNA: 3'- -GUGUCA--AGuUCCUUuGCUCUUA-CUGG- -5' |
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| 30722 | 5' | -45 | NC_006552.1 | + | 52345 | 1.15 | 0.006788 |
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Target: 5'- gCACAGUUCAAGGAAACGAGAAUGACCa -3' miRNA: 3'- -GUGUCAAGUUCCUUUGCUCUUACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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