Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30723 | 3' | -58.7 | NC_006552.1 | + | 8590 | 0.66 | 0.519455 |
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Target: 5'- --cGGCUUGGCCACCGc---CUUGUUg -3' miRNA: 3'- cuuCCGGACCGGUGGCuccuGAACGA- -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 19772 | 0.67 | 0.449449 |
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Target: 5'- -uGGGCCUGGaaCACCGAcGugUUGUa -3' miRNA: 3'- cuUCCGGACCg-GUGGCUcCugAACGa -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 54562 | 0.68 | 0.439871 |
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Target: 5'- cAAGGCCUGGUgAUCGuGGACaccGCc -3' miRNA: 3'- cUUCCGGACCGgUGGCuCCUGaa-CGa -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 15524 | 0.68 | 0.43702 |
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Target: 5'- -uGGGCCUGcuggaugucguccuGCaCGCCGAuuggcgucgGGGCUUGCUg -3' miRNA: 3'- cuUCCGGAC--------------CG-GUGGCU---------CCUGAACGA- -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 3580 | 0.68 | 0.402761 |
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Target: 5'- --uGGCC-GaGCCGCCGgcgccggcAGGACUUGCc -3' miRNA: 3'- cuuCCGGaC-CGGUGGC--------UCCUGAACGa -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 58192 | 0.7 | 0.333995 |
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Target: 5'- aGAAGGcCCUGGCCgaagccacgcugcGCCGGGuGGCUaaggGCg -3' miRNA: 3'- -CUUCC-GGACCGG-------------UGGCUC-CUGAa---CGa -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 54590 | 0.74 | 0.168631 |
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Target: 5'- --cGGCgaaGGCCGCCGAGGAUcUGCUg -3' miRNA: 3'- cuuCCGga-CCGGUGGCUCCUGaACGA- -5' |
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| 30723 | 3' | -58.7 | NC_006552.1 | + | 52189 | 1.07 | 0.000682 |
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Target: 5'- cGAAGGCCUGGCCACCGAGGACUUGCUc -3' miRNA: 3'- -CUUCCGGACCGGUGGCUCCUGAACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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