Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30725 | 3' | -57 | NC_006552.1 | + | 60294 | 0.67 | 0.592715 |
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Target: 5'- uGGCCCUGGGCaucGGCCccuGGGuguuccGCGAAa -3' miRNA: 3'- gUCGGGACCUGcu-UCGGu--UCC------UGCUU- -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 10327 | 0.67 | 0.560323 |
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Target: 5'- aAGCCCUGGcCGA-GCCGcccauGGCGAAa -3' miRNA: 3'- gUCGGGACCuGCUuCGGUuc---CUGCUU- -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 57614 | 0.69 | 0.457021 |
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Target: 5'- --aCCCUGacGACGAGuGCCAGGGACGc- -3' miRNA: 3'- gucGGGAC--CUGCUU-CGGUUCCUGCuu -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 52229 | 0.69 | 0.428024 |
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Target: 5'- cCGGCCCUGGACaaccuGGCCAacagcaAGGGCc-- -3' miRNA: 3'- -GUCGGGACCUGcu---UCGGU------UCCUGcuu -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 15584 | 0.7 | 0.391109 |
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Target: 5'- uGGUCCaGGGCGAAGCCGcgagcacGGGCGGc -3' miRNA: 3'- gUCGGGaCCUGCUUCGGUu------CCUGCUu -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 3744 | 0.7 | 0.390213 |
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Target: 5'- -uGUUCgcggGGGCGAcaucaauGGCCGAGGACGAGu -3' miRNA: 3'- guCGGGa---CCUGCU-------UCGGUUCCUGCUU- -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 57422 | 0.7 | 0.382211 |
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Target: 5'- uGGCCCUGGACGAcaCCGAcGACGc- -3' miRNA: 3'- gUCGGGACCUGCUucGGUUcCUGCuu -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 58195 | 0.75 | 0.184247 |
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Target: 5'- aGGCCCUGGcCGAAGCCAcgcugcgccGGGugGc- -3' miRNA: 3'- gUCGGGACCuGCUUCGGU---------UCCugCuu -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 49663 | 0.76 | 0.16101 |
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Target: 5'- gAGCCCgaGGACGAagAGCagGAGGACGAAg -3' miRNA: 3'- gUCGGGa-CCUGCU--UCGg-UUCCUGCUU- -5' |
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| 30725 | 3' | -57 | NC_006552.1 | + | 51059 | 1.06 | 0.001163 |
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Target: 5'- aCAGCCCUGGACGAAGCCAAGGACGAAu -3' miRNA: 3'- -GUCGGGACCUGCUUCGGUUCCUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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