Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30725 | 5' | -52.6 | NC_006552.1 | + | 7014 | 0.72 | 0.582922 |
Target: 5'- uGCgucUCGCGCACGGCcCGGCguGCGAacUCGu -3' miRNA: 3'- -CGa--AGCGCGUGUUGaGCUG--CGCU--AGC- -5' |
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30725 | 5' | -52.6 | NC_006552.1 | + | 9796 | 0.73 | 0.518745 |
Target: 5'- aUUUCGUGCAgCAACacugcCGGCGCGGUCa -3' miRNA: 3'- cGAAGCGCGU-GUUGa----GCUGCGCUAGc -5' |
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30725 | 5' | -52.6 | NC_006552.1 | + | 43541 | 0.75 | 0.418971 |
Target: 5'- cGUcUCGCGCggcgucccgaACAGCuuUCGACGCGAUCu -3' miRNA: 3'- -CGaAGCGCG----------UGUUG--AGCUGCGCUAGc -5' |
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30725 | 5' | -52.6 | NC_006552.1 | + | 2713 | 0.75 | 0.409639 |
Target: 5'- cGCUUCuucaGCGCAGCUUGGCGCcAUCGc -3' miRNA: 3'- -CGAAGcg--CGUGUUGAGCUGCGcUAGC- -5' |
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30725 | 5' | -52.6 | NC_006552.1 | + | 51096 | 1.1 | 0.002193 |
Target: 5'- gGCUUCGCGCACAACUCGACGCGAUCGc -3' miRNA: 3'- -CGAAGCGCGUGUUGAGCUGCGCUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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