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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30727 | 5' | -57.3 | NC_006552.1 | + | 15974 | 0.66 | 0.607156 |
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Target: 5'- cCUCGgcaGCCugCUGGAGCGcGGCcUGCUGc -3' miRNA: 3'- -GAGUag-CGG--GACCUCGU-CCGcAUGAC- -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 51135 | 0.67 | 0.574905 |
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Target: 5'- aCUCccgCGCCCUGGAGUuaauGCGUAa-- -3' miRNA: 3'- -GAGua-GCGGGACCUCGuc--CGCAUgac -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 24048 | 0.67 | 0.553618 |
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Target: 5'- uUCAUCGCCCaGccGCAGGcCGUAUa- -3' miRNA: 3'- gAGUAGCGGGaCcuCGUCC-GCAUGac -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 46706 | 0.67 | 0.553618 |
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Target: 5'- aUgGUCGCUugccugauCUGGAGCAGGCGa---- -3' miRNA: 3'- gAgUAGCGG--------GACCUCGUCCGCaugac -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 28057 | 0.67 | 0.543061 |
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Target: 5'- gCUUGUCGCCCauccuGAGCAGGUugGUGcCUGg -3' miRNA: 3'- -GAGUAGCGGGac---CUCGUCCG--CAU-GAC- -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 32115 | 0.72 | 0.313036 |
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Target: 5'- --uGUCGCCCUGGAu--GGCGUACa- -3' miRNA: 3'- gagUAGCGGGACCUcguCCGCAUGac -5' |
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| 30727 | 5' | -57.3 | NC_006552.1 | + | 50150 | 1.08 | 0.000878 |
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Target: 5'- aCUCAUCGCCCUGGAGCAGGCGUACUGc -3' miRNA: 3'- -GAGUAGCGGGACCUCGUCCGCAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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