Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30728 | 5' | -54.5 | NC_006552.1 | + | 39289 | 0.66 | 0.772042 |
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Target: 5'- uGCCGGcGCAGGAAGCGgcaggccuccCAGUCgucgccGCAc -3' miRNA: 3'- gUGGCU-UGUCCUUCGC----------GUCAGa-----CGUu -5' |
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| 30728 | 5' | -54.5 | NC_006552.1 | + | 5385 | 0.66 | 0.740734 |
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Target: 5'- aCGCCGGGCAGcGAGuCGCGGaaCUGCu- -3' miRNA: 3'- -GUGGCUUGUCcUUC-GCGUCa-GACGuu -5' |
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| 30728 | 5' | -54.5 | NC_006552.1 | + | 15239 | 0.66 | 0.740734 |
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Target: 5'- aCGCUGAccuuCGGGAaaGGCGUcGUCUGCc- -3' miRNA: 3'- -GUGGCUu---GUCCU--UCGCGuCAGACGuu -5' |
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| 30728 | 5' | -54.5 | NC_006552.1 | + | 21448 | 0.71 | 0.47258 |
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Target: 5'- gGCCGAACuuGAAGCGCccauuggucaacucGUCUGCAGg -3' miRNA: 3'- gUGGCUUGucCUUCGCGu-------------CAGACGUU- -5' |
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| 30728 | 5' | -54.5 | NC_006552.1 | + | 38231 | 0.74 | 0.298855 |
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Target: 5'- uCAUUGGACAGGuaggcGGCGcCGGUCUGCGGa -3' miRNA: 3'- -GUGGCUUGUCCu----UCGC-GUCAGACGUU- -5' |
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| 30728 | 5' | -54.5 | NC_006552.1 | + | 50053 | 1.06 | 0.001916 |
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Target: 5'- cCACCGAACAGGAAGCGCAGUCUGCAAu -3' miRNA: 3'- -GUGGCUUGUCCUUCGCGUCAGACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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