miRNA: 3'- uCGCGGUGu-UGG-CGCGUCGca--AGUUg -5'
miRNA: 3'- uCGCGGUGu-----UG-GCGCGuCg---CAAGUUG- -5'
miRNA: 3'- uCGCGGUGu-UGGCgCGUCg-CA-----AGUUG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30729 | 3' | -55.8 | NC_006552.1 | + | 63556 | 0.7 | 0.455681 |
|
Target: 5'- uGCGCCACccGCCuGCGCGGCaucgUCGAa -3' miRNA: 3'- uCGCGGUGu-UGG-CGCGUCGca--AGUUg -5' |
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| 30729 | 3' | -55.8 | NC_006552.1 | + | 64154 | 0.67 | 0.635628 |
|
Target: 5'- cGCGCCGCccuggcGCaCGCGCcGaccaGUUCGGCa -3' miRNA: 3'- uCGCGGUGu-----UG-GCGCGuCg---CAAGUUG- -5' |
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| 30729 | 3' | -55.8 | NC_006552.1 | + | 65035 | 0.67 | 0.668466 |
|
Target: 5'- cGCGCCACucGCUGgGCAGagGUugaaaUCGACc -3' miRNA: 3'- uCGCGGUGu-UGGCgCGUCg-CA-----AGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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