Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30730 | 5' | -58 | NC_006552.1 | + | 28993 | 0.66 | 0.639265 |
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Target: 5'- uUGAGgcgcgACCGUCUGCgacucgggaaugaUCCGCGug-CCGa -3' miRNA: 3'- -ACUCa----UGGCAGGCG-------------AGGCGCugaGGU- -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 64002 | 0.66 | 0.619042 |
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Target: 5'- aGGGccugGCCGgcacaCCGCUUCGUGACgcgCCu -3' miRNA: 3'- aCUCa---UGGCa----GGCGAGGCGCUGa--GGu -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 22217 | 0.67 | 0.545296 |
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Target: 5'- aGGGUGCCGgugacgcgaUCCGCUgCCGUGGCcguaucgaCCAc -3' miRNA: 3'- aCUCAUGGC---------AGGCGA-GGCGCUGa-------GGU- -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 3775 | 0.67 | 0.54426 |
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Target: 5'- cGAGUgACCGUCCGCgcagCCuugauccgaaaauGUGACcuuUCCAg -3' miRNA: 3'- aCUCA-UGGCAGGCGa---GG-------------CGCUG---AGGU- -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 53117 | 0.68 | 0.504391 |
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Target: 5'- -aAGuUGCCGcUCUGCUCUGCGAUgccgCCGg -3' miRNA: 3'- acUC-AUGGC-AGGCGAGGCGCUGa---GGU- -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 42075 | 0.7 | 0.391058 |
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Target: 5'- -cGGUGaucugCGUCCGCUCCuGCuGCUCCAg -3' miRNA: 3'- acUCAUg----GCAGGCGAGG-CGcUGAGGU- -5' |
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| 30730 | 5' | -58 | NC_006552.1 | + | 48774 | 1.08 | 0.000828 |
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Target: 5'- uUGAGUACCGUCCGCUCCGCGACUCCAu -3' miRNA: 3'- -ACUCAUGGCAGGCGAGGCGCUGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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