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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30734 | 5' | -54 | NC_006552.1 | + | 41843 | 0.67 | 0.741617 |
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Target: 5'- aGGGugcGGcucGGCAUugGGGucgAAGCCugGGCc -3' miRNA: 3'- -CCCu--CC---CCGUAugCCC---UUUGGugUUG- -5' |
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| 30734 | 5' | -54 | NC_006552.1 | + | 50263 | 0.69 | 0.666554 |
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Target: 5'- aGGGAGGGGCcucCGcauGAAGCCaaauGCGACu -3' miRNA: 3'- -CCCUCCCCGuauGCc--CUUUGG----UGUUG- -5' |
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| 30734 | 5' | -54 | NC_006552.1 | + | 57471 | 0.69 | 0.644655 |
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Target: 5'- cGGAGGuGGCgccgGUACGGGcuuggaaauGGGCCugGGCc -3' miRNA: 3'- cCCUCC-CCG----UAUGCCC---------UUUGGugUUG- -5' |
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| 30734 | 5' | -54 | NC_006552.1 | + | 33707 | 0.7 | 0.56707 |
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Target: 5'- cGGc-GGGCA-ACGGGAAcucgaacGCCACGACa -3' miRNA: 3'- cCCucCCCGUaUGCCCUU-------UGGUGUUG- -5' |
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| 30734 | 5' | -54 | NC_006552.1 | + | 47767 | 1.13 | 0.000879 |
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Target: 5'- cGGGAGGGGCAUACGGGAAACCACAACg -3' miRNA: 3'- -CCCUCCCCGUAUGCCCUUUGGUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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