Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30735 | 3' | -53.4 | NC_006552.1 | + | 19728 | 0.67 | 0.78216 |
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Target: 5'- cGUcgGcCACGuuggcgcuGGCCGcUGCGCGCCa- -3' miRNA: 3'- -CGuaCaGUGCu-------UCGGU-ACGCGUGGaa -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 26097 | 0.67 | 0.76161 |
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Target: 5'- gGCAUcugCACGAAGCCGUaGCGCucAUCg- -3' miRNA: 3'- -CGUAca-GUGCUUCGGUA-CGCG--UGGaa -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 41191 | 0.67 | 0.76161 |
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Target: 5'- aCAUGaUCGCGAucagcgucguaaAGCCGagcUGaCGCGCCUUg -3' miRNA: 3'- cGUAC-AGUGCU------------UCGGU---AC-GCGUGGAA- -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 8507 | 0.68 | 0.697039 |
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Target: 5'- cGCAUGgcgCGCucAGCCAgcgaaucgacGCGCACCg- -3' miRNA: 3'- -CGUACa--GUGcuUCGGUa---------CGCGUGGaa -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 40977 | 0.68 | 0.674865 |
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Target: 5'- cGCAUGg-ACGuGGCCAcGCGCACaCUg -3' miRNA: 3'- -CGUACagUGCuUCGGUaCGCGUG-GAa -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 47845 | 0.69 | 0.652523 |
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Target: 5'- cGCGUggcGUCGCGAAGgaaggcaaguuCC-UGCGCACCa- -3' miRNA: 3'- -CGUA---CAGUGCUUC-----------GGuACGCGUGGaa -5' |
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| 30735 | 3' | -53.4 | NC_006552.1 | + | 45983 | 1.07 | 0.002167 |
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Target: 5'- uGCAUGUCACGAAGCCAUGCGCACCUUu -3' miRNA: 3'- -CGUACAGUGCUUCGGUACGCGUGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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