Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30735 | 5' | -57.9 | NC_006552.1 | + | 65018 | 0.66 | 0.617549 |
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Target: 5'- -aAGGGCGCaaguuuggccGCGCCacUCgCUgGGCAGAGg -3' miRNA: 3'- ccUUCCGCG----------CGUGG--AG-GAgUCGUCUC- -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 26164 | 0.67 | 0.57483 |
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Target: 5'- cGuuGGCGCGCGCga--UCAGCAGAc -3' miRNA: 3'- cCuuCCGCGCGUGgaggAGUCGUCUc -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 64278 | 0.67 | 0.543231 |
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Target: 5'- aGAAcGGCGCGCugUUCUgucaccacugCGGCGGAc -3' miRNA: 3'- cCUU-CCGCGCGugGAGGa---------GUCGUCUc -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 25922 | 0.69 | 0.44304 |
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Target: 5'- cGuuGGUGCcUACCaCCUCGGCAGGGa -3' miRNA: 3'- cCuuCCGCGcGUGGaGGAGUCGUCUC- -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 33530 | 0.69 | 0.44304 |
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Target: 5'- uGGAacuggugcgcAGGCGCGCAgCggCCgacagCAGCAGGc -3' miRNA: 3'- -CCU----------UCCGCGCGUgGa-GGa----GUCGUCUc -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 35890 | 0.69 | 0.42425 |
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Target: 5'- ---uGGCGCGCGCUgcugCCUCAucccGCAGGc -3' miRNA: 3'- ccuuCCGCGCGUGGa---GGAGU----CGUCUc -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 26346 | 0.71 | 0.337115 |
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Target: 5'- aGGuaGAGGCGCGCGCCggucacacuggcaUCCaagcaCAGCGGAa -3' miRNA: 3'- -CC--UUCCGCGCGUGG-------------AGGa----GUCGUCUc -5' |
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| 30735 | 5' | -57.9 | NC_006552.1 | + | 46017 | 1.1 | 0.00058 |
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Target: 5'- gGGAAGGCGCGCACCUCCUCAGCAGAGu -3' miRNA: 3'- -CCUUCCGCGCGUGGAGGAGUCGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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