Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30743 | 5' | -53.9 | NC_006552.1 | + | 26625 | 0.66 | 0.852736 |
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Target: 5'- aGCAccUCGUgGGaCaGGU-CCCCUUCGCc -3' miRNA: 3'- -CGU--AGCAgCCaGgUCAuGGGGAAGUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 20507 | 0.66 | 0.826573 |
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Target: 5'- -uGUCG-CGGUUCAGauucgGCCgCCUUCAg -3' miRNA: 3'- cgUAGCaGCCAGGUCa----UGG-GGAAGUg -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 57185 | 0.7 | 0.619562 |
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Target: 5'- gGCAUgGU-GGUCCucucugGGUACCCCUgccCGCu -3' miRNA: 3'- -CGUAgCAgCCAGG------UCAUGGGGAa--GUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 30793 | 0.7 | 0.608633 |
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Target: 5'- cGUuUCGUCuGG-CCAGUAgCCUUUCGCc -3' miRNA: 3'- -CGuAGCAG-CCaGGUCAUgGGGAAGUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 56480 | 0.7 | 0.597724 |
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Target: 5'- gGCAgggCGgc-GUCCAGUGCCUgUUCACc -3' miRNA: 3'- -CGUa--GCagcCAGGUCAUGGGgAAGUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 19275 | 0.73 | 0.451439 |
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Target: 5'- -uGUCGUCGGagguUCCGGU-CCCCgggccagUCACg -3' miRNA: 3'- cgUAGCAGCC----AGGUCAuGGGGa------AGUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 13674 | 0.73 | 0.432081 |
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Target: 5'- --cUCGUCGGuacgggcgUCCAGUGCCgCCUggcgCACg -3' miRNA: 3'- cguAGCAGCC--------AGGUCAUGG-GGAa---GUG- -5' |
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| 30743 | 5' | -53.9 | NC_006552.1 | + | 43027 | 1.11 | 0.00139 |
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Target: 5'- gGCAUCGUCGGUCCAGUACCCCUUCACc -3' miRNA: 3'- -CGUAGCAGCCAGGUCAUGGGGAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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