Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30744 | 3' | -55.6 | NC_006552.1 | + | 17368 | 0.66 | 0.742678 |
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Target: 5'- aCGGGUUGcgccGACUGCGuuUGCUGGU-CGAu -3' miRNA: 3'- -GCCUAGC----UUGACGCc-ACGGCCAuGCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 32123 | 0.67 | 0.711647 |
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Target: 5'- uGGAUgGcguACaGCGGcuUGCCGGUGCa- -3' miRNA: 3'- gCCUAgCu--UGaCGCC--ACGGCCAUGcu -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 41235 | 0.67 | 0.690569 |
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Target: 5'- aGGAUCaGGUUGCGGUGCCauagGCGAc -3' miRNA: 3'- gCCUAGcUUGACGCCACGGcca-UGCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 13299 | 0.67 | 0.67888 |
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Target: 5'- gGGAUCGGGCU-UGGgugccucUGCCGGUuCGGa -3' miRNA: 3'- gCCUAGCUUGAcGCC-------ACGGCCAuGCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 28711 | 0.69 | 0.583539 |
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Target: 5'- uCGGccuggcauuguuGUCGAuCUGCGGgaagccgGCCGGcgGCGAa -3' miRNA: 3'- -GCC------------UAGCUuGACGCCa------CGGCCa-UGCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 25294 | 0.7 | 0.510328 |
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Target: 5'- uGGAcCGggUaGCGGUGUCGGU-CGGc -3' miRNA: 3'- gCCUaGCuuGaCGCCACGGCCAuGCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 31491 | 0.7 | 0.510328 |
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Target: 5'- uGGAUCGcaguCUGCGGcguguaugcGCCGGUAuCGAc -3' miRNA: 3'- gCCUAGCuu--GACGCCa--------CGGCCAU-GCU- -5' |
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| 30744 | 3' | -55.6 | NC_006552.1 | + | 42716 | 1.09 | 0.0013 |
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Target: 5'- gCGGAUCGAACUGCGGUGCCGGUACGAa -3' miRNA: 3'- -GCCUAGCUUGACGCCACGGCCAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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