Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30745 | 3' | -50.1 | NC_006552.1 | + | 11956 | 0.66 | 0.953471 |
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Target: 5'- ---gGUCGGagCGCaugGCGGugUGCUGGg -3' miRNA: 3'- gcuaUAGCUa-GCGa--CGCUugACGACU- -5' |
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| 30745 | 3' | -50.1 | NC_006552.1 | + | 8919 | 0.66 | 0.948491 |
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Target: 5'- aGGUAccUCGGUUGCcaacgGCGGACUugaccuguuccggGCUGAa -3' miRNA: 3'- gCUAU--AGCUAGCGa----CGCUUGA-------------CGACU- -5' |
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| 30745 | 3' | -50.1 | NC_006552.1 | + | 35454 | 0.67 | 0.933714 |
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Target: 5'- gCGu--UCGGcugcUGCUGCGA-CUGCUGAg -3' miRNA: 3'- -GCuauAGCUa---GCGACGCUuGACGACU- -5' |
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| 30745 | 3' | -50.1 | NC_006552.1 | + | 8389 | 0.68 | 0.909346 |
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Target: 5'- gCGGcUGUCGAUCaucuugGCGAACUGCUcGGg -3' miRNA: 3'- -GCU-AUAGCUAGcga---CGCUUGACGA-CU- -5' |
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| 30745 | 3' | -50.1 | NC_006552.1 | + | 55693 | 0.75 | 0.527263 |
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Target: 5'- aGggGUCGAUCGCcggGCGAGacgcgcacccCUGCUGAa -3' miRNA: 3'- gCuaUAGCUAGCGa--CGCUU----------GACGACU- -5' |
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| 30745 | 3' | -50.1 | NC_006552.1 | + | 42565 | 1.1 | 0.0034 |
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Target: 5'- cCGAUAUCGAUCGCUGCGAACUGCUGAg -3' miRNA: 3'- -GCUAUAGCUAGCGACGCUUGACGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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