Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30745 | 5' | -56 | NC_006552.1 | + | 36179 | 0.71 | 0.383934 |
Target: 5'- uCGCCAGCA--UCGC-CGGCAcCCGCg -3' miRNA: 3'- -GCGGUUGUcaAGCGcGUCGUaGGCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 60544 | 0.71 | 0.392853 |
Target: 5'- gGCCAGCAccGUUCGC-CGGCGacucgCCGCa -3' miRNA: 3'- gCGGUUGU--CAAGCGcGUCGUa----GGCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 50097 | 0.71 | 0.411089 |
Target: 5'- aCGCgGGCGGaaCGCGCAGCA-CgGCg -3' miRNA: 3'- -GCGgUUGUCaaGCGCGUCGUaGgCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 22056 | 0.71 | 0.420401 |
Target: 5'- gGCCAguGCuGUcgcugaugCGCGCGGCAUCCaGCa -3' miRNA: 3'- gCGGU--UGuCAa-------GCGCGUCGUAGG-CGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 12220 | 0.7 | 0.439403 |
Target: 5'- gGCCu-CAGcgCGgGCGGcCAUCCGCg -3' miRNA: 3'- gCGGuuGUCaaGCgCGUC-GUAGGCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 12703 | 0.69 | 0.509484 |
Target: 5'- aGCCucCAGUUCGuCGUAGCGaauaCGCUg -3' miRNA: 3'- gCGGuuGUCAAGC-GCGUCGUag--GCGA- -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 41408 | 0.69 | 0.530379 |
Target: 5'- aGCCAGgcagcgcgcCAGUUCG-GCcGCGUCCGUg -3' miRNA: 3'- gCGGUU---------GUCAAGCgCGuCGUAGGCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 38365 | 0.69 | 0.530379 |
Target: 5'- uGCCAgcaGCAGUUCGCccacCAGgGUCCGg- -3' miRNA: 3'- gCGGU---UGUCAAGCGc---GUCgUAGGCga -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 42014 | 0.68 | 0.551579 |
Target: 5'- uGCgu-CAGUUCGCGU-GCAUCUGCc -3' miRNA: 3'- gCGguuGUCAAGCGCGuCGUAGGCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 44186 | 0.68 | 0.561204 |
Target: 5'- gCGCCGACGGUccaugucuUCGCGCucguggcgcagguGGCgAUCaCGCa -3' miRNA: 3'- -GCGGUUGUCA--------AGCGCG-------------UCG-UAG-GCGa -5' |
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30745 | 5' | -56 | NC_006552.1 | + | 32350 | 0.66 | 0.724481 |
Target: 5'- gCGCaCGGCGGcUUCGCGCAcuGCGU-CGUUg -3' miRNA: 3'- -GCG-GUUGUC-AAGCGCGU--CGUAgGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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