miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30748 5' -52.3 NC_006552.1 + 61114 0.66 0.895507
Target:  5'- uCGAGCGgcuGCAGgaCAUCagcgaagagcagGCGUUGGCa -3'
miRNA:   3'- -GCUUGU---CGUCaaGUAGg-----------CGCAACCGa -5'
30748 5' -52.3 NC_006552.1 + 38102 0.67 0.840815
Target:  5'- aGGACAGCAGccUgGUaCUGCGgcGGCa -3'
miRNA:   3'- gCUUGUCGUCa-AgUA-GGCGCaaCCGa -5'
30748 5' -52.3 NC_006552.1 + 5967 0.69 0.752028
Target:  5'- aGAACGGCaaauccuuacaggGGUUCAgUCCaGCGUUGcGCg -3'
miRNA:   3'- gCUUGUCG-------------UCAAGU-AGG-CGCAAC-CGa -5'
30748 5' -52.3 NC_006552.1 + 44947 0.69 0.731888
Target:  5'- gGAaaGCAGCAGUUCGUCUuguugcucagGCGUcagcaugGGCa -3'
miRNA:   3'- gCU--UGUCGUCAAGUAGG----------CGCAa------CCGa -5'
30748 5' -52.3 NC_006552.1 + 1209 0.73 0.512105
Target:  5'- gCGGACAGCAGU--AUCCGUGagcGGCa -3'
miRNA:   3'- -GCUUGUCGUCAagUAGGCGCaa-CCGa -5'
30748 5' -52.3 NC_006552.1 + 41016 1.09 0.002393
Target:  5'- gCGAACAGCAGUUCAUCCGCGUUGGCUg -3'
miRNA:   3'- -GCUUGUCGUCAAGUAGGCGCAACCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.