Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30750 | 5' | -56.6 | NC_006552.1 | + | 2592 | 0.66 | 0.702185 |
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Target: 5'- gACGCUaCGGCuCGAagGCGauGCGCUCGUa -3' miRNA: 3'- aUGCGA-GCUGcGCU--CGC--UGCGAGUAc -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 40447 | 0.66 | 0.702185 |
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Target: 5'- uUGCGCUUG-CGC-AGCGuCGC-CAUGu -3' miRNA: 3'- -AUGCGAGCuGCGcUCGCuGCGaGUAC- -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 42749 | 0.66 | 0.691538 |
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Target: 5'- cGCGCUCGACGCGcAGCaccaGCaagUCGUc -3' miRNA: 3'- aUGCGAGCUGCGC-UCGcug-CG---AGUAc -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 7064 | 0.66 | 0.670088 |
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Target: 5'- cUGCGCUuuggCGAUGCGAuacgacGCGAUGUaggUCAUGc -3' miRNA: 3'- -AUGCGA----GCUGCGCU------CGCUGCG---AGUAC- -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 64734 | 0.69 | 0.499668 |
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Target: 5'- gACGUggCGGCGCugcaGGUGGCGCUCAa- -3' miRNA: 3'- aUGCGa-GCUGCGc---UCGCUGCGAGUac -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 60058 | 0.7 | 0.430823 |
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Target: 5'- cUGCGCUCGAgGCcuGCGGCGgUCGg- -3' miRNA: 3'- -AUGCGAGCUgCGcuCGCUGCgAGUac -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 44206 | 0.71 | 0.36786 |
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Target: 5'- cGCGCUCGugGCGcaGGUGGCGaUCAc- -3' miRNA: 3'- aUGCGAGCugCGC--UCGCUGCgAGUac -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 5673 | 0.72 | 0.351069 |
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Target: 5'- gGC-CUCGAUGCGGGCaGCGCUCu-- -3' miRNA: 3'- aUGcGAGCUGCGCUCGcUGCGAGuac -5' |
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| 30750 | 5' | -56.6 | NC_006552.1 | + | 38705 | 1.06 | 0.001519 |
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Target: 5'- gUACGCUCGACGCGAGCGACGCUCAUGc -3' miRNA: 3'- -AUGCGAGCUGCGCUCGCUGCGAGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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