Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30751 | 5' | -63.1 | NC_006552.1 | + | 17106 | 0.66 | 0.387471 |
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Target: 5'- gCCGCUuccggaaCCCcaaUCaCCCCCUUGGCCACg- -3' miRNA: 3'- -GGUGAc------GGG---AG-GGGGGGAUCGGUGga -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 8565 | 0.66 | 0.361841 |
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Target: 5'- cCCACaucaacgUGCCCUCaaauaCCggCUUGGCCACCg -3' miRNA: 3'- -GGUG-------ACGGGAGg----GGg-GGAUCGGUGGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 34934 | 0.66 | 0.354624 |
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Target: 5'- gCCAgUGCCCUUgCCCUCggcguacucGCUACCc -3' miRNA: 3'- -GGUgACGGGAGgGGGGGau-------CGGUGGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 16431 | 0.67 | 0.323791 |
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Target: 5'- gCCGCUcGCCCagUCCCgCCCC-AG-CGCCa -3' miRNA: 3'- -GGUGA-CGGG--AGGG-GGGGaUCgGUGGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 47087 | 0.67 | 0.30914 |
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Target: 5'- aCACUgaGCCCgcucCCaCCCgCCUGGCCAgCCc -3' miRNA: 3'- gGUGA--CGGGa---GG-GGG-GGAUCGGU-GGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 60593 | 0.67 | 0.302006 |
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Target: 5'- gCGCUGCUCaCCCCacaCCUA-CCGCCg -3' miRNA: 3'- gGUGACGGGaGGGGg--GGAUcGGUGGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 16369 | 0.68 | 0.281368 |
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Target: 5'- aCCGCcGCCggCCCgCCCaUAGCCAUCc -3' miRNA: 3'- -GGUGaCGGgaGGGgGGG-AUCGGUGGa -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 64540 | 0.69 | 0.226174 |
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Target: 5'- gCAUcGCCCUUCCCCgCUGGCU-CCUu -3' miRNA: 3'- gGUGaCGGGAGGGGGgGAUCGGuGGA- -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 46131 | 0.73 | 0.119075 |
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Target: 5'- uCCGCUGCCUUCgCCugCCCCUgcucGGCCACa- -3' miRNA: 3'- -GGUGACGGGAG-GG--GGGGA----UCGGUGga -5' |
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| 30751 | 5' | -63.1 | NC_006552.1 | + | 37512 | 1.08 | 0.000281 |
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Target: 5'- uCCACUGCCCUCCCCCCCUAGCCACCUc -3' miRNA: 3'- -GGUGACGGGAGGGGGGGAUCGGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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