Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30752 | 3' | -59.4 | NC_006552.1 | + | 15575 | 0.66 | 0.4913 |
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Target: 5'- uGCGCCGCCUGGUCCa-GGGCgaagCCg- -3' miRNA: 3'- cUGCGGUGGGUCAGGggCUUGa---GGaa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 45668 | 0.67 | 0.471499 |
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Target: 5'- cGAC-CCGUCCAGUUUCCGAuuccACUCCUg -3' miRNA: 3'- -CUGcGGUGGGUCAGGGGCU----UGAGGAa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 14629 | 0.67 | 0.471499 |
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Target: 5'- -uCGCCACCUuaUCCCggcUGAACUCCg- -3' miRNA: 3'- cuGCGGUGGGucAGGG---GCUUGAGGaa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 63699 | 0.67 | 0.461751 |
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Target: 5'- --aGCCGCCCAGgaCCUGGcACUCCg- -3' miRNA: 3'- cugCGGUGGGUCagGGGCU-UGAGGaa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 31705 | 0.67 | 0.433164 |
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Target: 5'- cGCGCCACCUg---CCCGGACUUCUUu -3' miRNA: 3'- cUGCGGUGGGucagGGGCUUGAGGAA- -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 64773 | 0.67 | 0.433164 |
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Target: 5'- cGCGCCACCgAcgCCCUGGGCaUCCUg -3' miRNA: 3'- cUGCGGUGGgUcaGGGGCUUG-AGGAa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 14808 | 0.68 | 0.387905 |
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Target: 5'- aACuCCGCCCAGccuUCgCCGAugUCCUUg -3' miRNA: 3'- cUGcGGUGGGUC---AGgGGCUugAGGAA- -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 60281 | 0.7 | 0.307196 |
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Target: 5'- cGCGCCACCCGGcuggCCCUGGGCaUCg-- -3' miRNA: 3'- cUGCGGUGGGUCa---GGGGCUUG-AGgaa -5' |
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| 30752 | 3' | -59.4 | NC_006552.1 | + | 35585 | 1.05 | 0.000842 |
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Target: 5'- gGACGCCACCCAGUCCCCGAACUCCUUg -3' miRNA: 3'- -CUGCGGUGGGUCAGGGGCUUGAGGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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