Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
30752 | 5' | -59.5 | NC_006552.1 | + | 2634 | 0.66 | 0.541852 |
Target: 5'- uCGAUGGGGcUGGCGgcccuUCUGGGcuaGGGaCCg -3' miRNA: 3'- -GUUACCUCaGCCGC-----AGGCCCa--CCC-GG- -5' |
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30752 | 5' | -59.5 | NC_006552.1 | + | 64801 | 0.67 | 0.521576 |
Target: 5'- ---cGGAaaCGGCGuuUCCGGccGGGCCg -3' miRNA: 3'- guuaCCUcaGCCGC--AGGCCcaCCCGG- -5' |
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30752 | 5' | -59.5 | NC_006552.1 | + | 25305 | 0.69 | 0.407533 |
Target: 5'- gCGGUGucGGUCGGCGUCCaagcuGGGcGGGUa -3' miRNA: 3'- -GUUACc-UCAGCCGCAGG-----CCCaCCCGg -5' |
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30752 | 5' | -59.5 | NC_006552.1 | + | 35619 | 1.1 | 0.000429 |
Target: 5'- gCAAUGGAGUCGGCGUCCGGGUGGGCCu -3' miRNA: 3'- -GUUACCUCAGCCGCAGGCCCACCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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