Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30753 | 5' | -57.3 | NC_006552.1 | + | 59630 | 0.66 | 0.66177 |
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Target: 5'- aCUCGGGcCGGgaggUGCUGGCUGGGGaCUu -3' miRNA: 3'- -GAGCUUaGCCa---ACGGUCGGCCUCcGA- -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 8914 | 0.66 | 0.647793 |
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Target: 5'- gUCGAAgguaccUCGGUUGCCAacGgCGGAcuugaccuguuccgGGCUg -3' miRNA: 3'- gAGCUU------AGCCAACGGU--CgGCCU--------------CCGA- -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 50326 | 0.67 | 0.597216 |
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Target: 5'- gUCGGcaAUCGGcgUGCUGGCCcuGGuGGCUc -3' miRNA: 3'- gAGCU--UAGCCa-ACGGUCGG--CCuCCGA- -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 21368 | 0.67 | 0.572638 |
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Target: 5'- uUCGAcgCucaGGUcauugauauagccgUGCCGGCCGGucAGGCg -3' miRNA: 3'- gAGCUuaG---CCA--------------ACGGUCGGCC--UCCGa -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 608 | 0.69 | 0.434009 |
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Target: 5'- --aGAAUCGGauugUGCuCAGUCGGcGGCUg -3' miRNA: 3'- gagCUUAGCCa---ACG-GUCGGCCuCCGA- -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 28727 | 0.73 | 0.264888 |
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Target: 5'- gUCGAucugCGGgaaGCCGGCCGGcGGCg -3' miRNA: 3'- gAGCUua--GCCaa-CGGUCGGCCuCCGa -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 18571 | 0.73 | 0.25833 |
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Target: 5'- -cCGAGUCGGUUcGCCAGCC--AGGCc -3' miRNA: 3'- gaGCUUAGCCAA-CGGUCGGccUCCGa -5' |
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| 30753 | 5' | -57.3 | NC_006552.1 | + | 35341 | 1.07 | 0.001049 |
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Target: 5'- cCUCGAAUCGGUUGCCAGCCGGAGGCUu -3' miRNA: 3'- -GAGCUUAGCCAACGGUCGGCCUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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