Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30760 | 3' | -54.6 | NC_006552.1 | + | 15499 | 0.66 | 0.767065 |
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Target: 5'- cGCCU--GCUGCgCGUCAGCCUUGc- -3' miRNA: 3'- aCGGGcuCGGUGaGUAGUCGGAAUac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 4958 | 0.66 | 0.756762 |
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Target: 5'- aGCUCG-GCCAgCUUGgccUCAGCCUUGg- -3' miRNA: 3'- aCGGGCuCGGU-GAGU---AGUCGGAAUac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 902 | 0.66 | 0.742123 |
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Target: 5'- cGCCCcuguuucaacuccAGCCACUguUCAGCCacGUGu -3' miRNA: 3'- aCGGGc------------UCGGUGAguAGUCGGaaUAC- -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 19294 | 0.68 | 0.65962 |
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Target: 5'- -cCCCGGGCCAgUCAcgcgcccaccuUCAGCCa---- -3' miRNA: 3'- acGGGCUCGGUgAGU-----------AGUCGGaauac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 54018 | 0.68 | 0.65962 |
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Target: 5'- gGCCgCGAGCCGC-CAccgCGGCCg---- -3' miRNA: 3'- aCGG-GCUCGGUGaGUa--GUCGGaauac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 60077 | 0.68 | 0.615278 |
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Target: 5'- cGgUCGGGCCACUCuccagCAGCUcUAUGc -3' miRNA: 3'- aCgGGCUCGGUGAGua---GUCGGaAUAC- -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 41380 | 0.68 | 0.615278 |
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Target: 5'- aGCCgGAaaacaggcGCCACUCAgggUCAGCCa---- -3' miRNA: 3'- aCGGgCU--------CGGUGAGU---AGUCGGaauac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 19511 | 0.7 | 0.50666 |
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Target: 5'- cUGCCgGAGCCACUgAUacUAGCCg---- -3' miRNA: 3'- -ACGGgCUCGGUGAgUA--GUCGGaauac -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 24739 | 0.71 | 0.485831 |
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Target: 5'- gGCgCCGGGCCuGCUCGgUCAaCCUUAUGc -3' miRNA: 3'- aCG-GGCUCGG-UGAGU-AGUcGGAAUAC- -5' |
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| 30760 | 3' | -54.6 | NC_006552.1 | + | 31066 | 1.08 | 0.001459 |
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Target: 5'- uUGCCCGAGCCACUCAUCAGCCUUAUGc -3' miRNA: 3'- -ACGGGCUCGGUGAGUAGUCGGAAUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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