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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30762 | 3' | -57.5 | NC_006552.1 | + | 22218 | 0.69 | 0.466145 |
Target: 5'- gGGUGCCgGuGACGcGaUCCgcuGCCGUGGc -3' miRNA: 3'- gCCACGGgCuCUGC-CaAGG---UGGCAUC- -5' |
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30762 | 3' | -57.5 | NC_006552.1 | + | 28768 | 0.7 | 0.437661 |
Target: 5'- uGGUGCCCacuuGACGGUuucgguucUCCACCa--- -3' miRNA: 3'- gCCACGGGcu--CUGCCA--------AGGUGGcauc -5' |
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30762 | 3' | -57.5 | NC_006552.1 | + | 30117 | 1.09 | 0.00083 |
Target: 5'- aCGGUGCCCGAGACGGUUCCACCGUAGa -3' miRNA: 3'- -GCCACGGGCUCUGCCAAGGUGGCAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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