Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30762 | 5' | -53.9 | NC_006552.1 | + | 14061 | 0.66 | 0.817549 |
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Target: 5'- uCAUCGGcgGuUGGCGCC-CCCUGuguuGCGAu -3' miRNA: 3'- -GUGGUCa-C-ACUGUGGuGGGACu---UGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 57864 | 0.67 | 0.779015 |
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Target: 5'- uGgCAGUGUGGCGgCACCaggGAACa- -3' miRNA: 3'- gUgGUCACACUGUgGUGGga-CUUGcu -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 33248 | 0.68 | 0.738056 |
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Target: 5'- gCACuCGGcgaacgGUGGCGCCACCUUGucgccgauGGCGAg -3' miRNA: 3'- -GUG-GUCa-----CACUGUGGUGGGAC--------UUGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 28051 | 0.69 | 0.684551 |
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Target: 5'- cCACCAGcuUGUcGC-CCAUCCUGAGCa- -3' miRNA: 3'- -GUGGUC--ACAcUGuGGUGGGACUUGcu -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 56425 | 0.7 | 0.6188 |
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Target: 5'- gCACCg----GACGCCGCCCUGuggGCGAu -3' miRNA: 3'- -GUGGucacaCUGUGGUGGGACu--UGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 53994 | 0.7 | 0.607825 |
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Target: 5'- gGCCAGgcgcGUGGC-CCugGCCCUGGccGCGAg -3' miRNA: 3'- gUGGUCa---CACUGuGG--UGGGACU--UGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 57235 | 0.7 | 0.59687 |
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Target: 5'- cCGCCAc--UGGCACCGCCUcGAGCGGa -3' miRNA: 3'- -GUGGUcacACUGUGGUGGGaCUUGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 34621 | 0.71 | 0.56422 |
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Target: 5'- aCGCCAGUGaUGGCAUCGgCgCUGGcaACGAa -3' miRNA: 3'- -GUGGUCAC-ACUGUGGUgG-GACU--UGCU- -5' |
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| 30762 | 5' | -53.9 | NC_006552.1 | + | 30154 | 1.08 | 0.001872 |
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Target: 5'- uCACCAGUGUGACACCACCCUGAACGAu -3' miRNA: 3'- -GUGGUCACACUGUGGUGGGACUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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