Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30769 | 5' | -56.4 | NC_006552.1 | + | 28911 | 0.66 | 0.687367 |
Target: 5'- uGGACGGUgCUCuggaauCCGgCCGGCUCGg -3' miRNA: 3'- -CUUGCUAgGGGuu----GGCaGGCCGAGUu -5' |
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30769 | 5' | -56.4 | NC_006552.1 | + | 44407 | 0.66 | 0.676584 |
Target: 5'- cGGCGAUugcgcUgCCGGCCGgcgaaaCCGGCUCGAg -3' miRNA: 3'- cUUGCUA-----GgGGUUGGCa-----GGCCGAGUU- -5' |
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30769 | 5' | -56.4 | NC_006552.1 | + | 26635 | 0.67 | 0.633144 |
Target: 5'- gGGACaGGUCCCCuucGCCGaUCugugcgagCGGCUCGAa -3' miRNA: 3'- -CUUG-CUAGGGGu--UGGC-AG--------GCCGAGUU- -5' |
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30769 | 5' | -56.4 | NC_006552.1 | + | 45409 | 0.69 | 0.484494 |
Target: 5'- cGACGAauUCCCCGcCCGgggCCGGCgCAGc -3' miRNA: 3'- cUUGCU--AGGGGUuGGCa--GGCCGaGUU- -5' |
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30769 | 5' | -56.4 | NC_006552.1 | + | 44733 | 0.77 | 0.160475 |
Target: 5'- aGGGCGAUCgCCCAGgccUCGUUCGGCUCGGg -3' miRNA: 3'- -CUUGCUAG-GGGUU---GGCAGGCCGAGUU- -5' |
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30769 | 5' | -56.4 | NC_006552.1 | + | 27137 | 0.93 | 0.01285 |
Target: 5'- uGAACGAcUCCCAACCGUCCGGCUCAAc -3' miRNA: 3'- -CUUGCUaGGGGUUGGCAGGCCGAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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