Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30773 | 5' | -56.3 | NC_006552.1 | + | 43885 | 0.66 | 0.7113 |
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Target: 5'- uGCGGuGGACCAGG-CgGUGCUugCGa- -3' miRNA: 3'- -CGUC-CCUGGUCCuGaCGUGAugGUcc -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 20439 | 0.67 | 0.668742 |
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Target: 5'- -gAGGG-CCAGGcccuGCUGCACggcgaaGCgCAGGu -3' miRNA: 3'- cgUCCCuGGUCC----UGACGUGa-----UG-GUCC- -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 56717 | 0.67 | 0.668742 |
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Target: 5'- cGCGcGGGACCguggcgAGGaACUGC---GCCAGGc -3' miRNA: 3'- -CGU-CCCUGG------UCC-UGACGugaUGGUCC- -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 33177 | 0.67 | 0.651534 |
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Target: 5'- uCGGGGGuggugucuggguuguCCAGGuggagUUGCACgccgGCCAGGa -3' miRNA: 3'- cGUCCCU---------------GGUCCu----GACGUGa---UGGUCC- -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 44386 | 0.7 | 0.487857 |
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Target: 5'- cGgAGGGuCCAGucagcagcccggcGAUUGCGCUGCCGGc -3' miRNA: 3'- -CgUCCCuGGUC-------------CUGACGUGAUGGUCc -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 53899 | 0.73 | 0.319162 |
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Target: 5'- gGCAGGG-CCAGGACg-----GCCAGGa -3' miRNA: 3'- -CGUCCCuGGUCCUGacgugaUGGUCC- -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 61611 | 0.76 | 0.208252 |
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Target: 5'- aCAGGGACUGGGACgGCaACUGCCugaucuGGa -3' miRNA: 3'- cGUCCCUGGUCCUGaCG-UGAUGGu-----CC- -5' |
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| 30773 | 5' | -56.3 | NC_006552.1 | + | 25941 | 0.99 | 0.005619 |
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Target: 5'- gGCAGGGA-CAGGACUGCACUACCAGGc -3' miRNA: 3'- -CGUCCCUgGUCCUGACGUGAUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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