Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30778 | 3' | -53.6 | NC_006552.1 | + | 2999 | 0.66 | 0.843041 |
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Target: 5'- gGCGaCAacACCGGAGCGCGCg-GAg---- -3' miRNA: 3'- -CGC-GU--UGGCCUUGCGCGgaCUgaaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 18315 | 0.66 | 0.816019 |
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Target: 5'- gGCGCAgcACUGGAACuugGCGCCgcugcacccugGGCUg-- -3' miRNA: 3'- -CGCGU--UGGCCUUG---CGCGGa----------CUGAaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 32500 | 0.67 | 0.805662 |
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Target: 5'- cGCGCGuggacggcaucACgGGAGCGCGCCaagccgugcugcuUGACc--- -3' miRNA: 3'- -CGCGU-----------UGgCCUUGCGCGG-------------ACUGaaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 61720 | 0.67 | 0.797025 |
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Target: 5'- cGCGC-GCgGGuACGCGcCCUGGCc--- -3' miRNA: 3'- -CGCGuUGgCCuUGCGC-GGACUGaaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 50097 | 0.68 | 0.75706 |
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Target: 5'- aCGCGGgCGGAACGCGCagcacggcGGCgcgUUGa -3' miRNA: 3'- cGCGUUgGCCUUGCGCGga------CUGa--AAC- -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 59924 | 0.68 | 0.75706 |
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Target: 5'- gGCGC-GCCGGAcCGUGCUguccGGCUUc- -3' miRNA: 3'- -CGCGuUGGCCUuGCGCGGa---CUGAAac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 15978 | 0.68 | 0.725709 |
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Target: 5'- -gGCAGCCugcuGGAGCGCGgCCUG-CUg-- -3' miRNA: 3'- cgCGUUGG----CCUUGCGC-GGACuGAaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 21590 | 0.68 | 0.713987 |
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Target: 5'- gGCGCuAugCGGAugcaccaGCGCGcCCUGGCc--- -3' miRNA: 3'- -CGCG-UugGCCU-------UGCGC-GGACUGaaac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 19741 | 0.71 | 0.562253 |
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Target: 5'- gGCGCuGGCCGcuGCGCGCCaGGCUUc- -3' miRNA: 3'- -CGCG-UUGGCcuUGCGCGGaCUGAAac -5' |
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| 30778 | 3' | -53.6 | NC_006552.1 | + | 23791 | 1.1 | 0.001597 |
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Target: 5'- uGCGCAACCGGAACGCGCCUGACUUUGu -3' miRNA: 3'- -CGCGUUGGCCUUGCGCGGACUGAAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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