Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30782 | 3' | -56.6 | NC_006552.1 | + | 10840 | 0.66 | 0.680835 |
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Target: 5'- -cGCCGGGcUGCggCGcugccgacucuUCGGCUGCCGg -3' miRNA: 3'- caCGGCUU-ACGaaGC-----------AGCCGGUGGCa -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 64640 | 0.66 | 0.659305 |
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Target: 5'- -gGCCGAc-GCUguUCGcCGcGCCGCCGg -3' miRNA: 3'- caCGGCUuaCGA--AGCaGC-CGGUGGCa -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 21522 | 0.66 | 0.648499 |
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Target: 5'- cUGCCGcuuUGCgugguggucaCGUCcauGGCCACCGUg -3' miRNA: 3'- cACGGCuu-ACGaa--------GCAG---CCGGUGGCA- -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 42457 | 0.66 | 0.648499 |
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Target: 5'- -aGCUGg--GCUUCGUCG-CCAUCGg -3' miRNA: 3'- caCGGCuuaCGAAGCAGCcGGUGGCa -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 16086 | 0.67 | 0.626852 |
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Target: 5'- cUGCuCGGAgcGCUUUGg-GGCCGCCGg -3' miRNA: 3'- cACG-GCUUa-CGAAGCagCCGGUGGCa -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 38853 | 0.67 | 0.61603 |
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Target: 5'- aGUGCCG-GUGCguguacggCGUgGGaCCAUCGUg -3' miRNA: 3'- -CACGGCuUACGaa------GCAgCC-GGUGGCA- -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 5045 | 0.67 | 0.594437 |
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Target: 5'- gGUGCCGAuagGUaagCGUgcUGGCCACCa- -3' miRNA: 3'- -CACGGCUua-CGaa-GCA--GCCGGUGGca -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 63468 | 0.7 | 0.449941 |
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Target: 5'- uGUGCa-AAUGCUUCcgcuGcCGGCCGCCGg -3' miRNA: 3'- -CACGgcUUACGAAG----CaGCCGGUGGCa -5' |
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| 30782 | 3' | -56.6 | NC_006552.1 | + | 22111 | 1.07 | 0.001166 |
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Target: 5'- gGUGCCGAAUGCUUCGUCGGCCACCGUg -3' miRNA: 3'- -CACGGCUUACGAAGCAGCCGGUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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