Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30785 | 3' | -58.7 | NC_006552.1 | + | 63945 | 0.66 | 0.564242 |
Target: 5'- aGCGgcauGCCcCGGACAgcaaucugguGGCGC-GCGCCAc -3' miRNA: 3'- -CGC----UGGaGCUUGU----------CCGCGaCGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 34133 | 0.66 | 0.552656 |
Target: 5'- -aGugCUCGGAugccgccCAGGC-UUGCGCCAg -3' miRNA: 3'- cgCugGAGCUU-------GUCCGcGACGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 41226 | 0.66 | 0.53282 |
Target: 5'- cGCG-CCUUGAGgauCAGGuUGCgGUGCCAUa -3' miRNA: 3'- -CGCuGGAGCUU---GUCC-GCGaCGCGGUA- -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 38225 | 0.66 | 0.53282 |
Target: 5'- aGCaGAUCaUUGGACAGGUagGCgGCGCCGg -3' miRNA: 3'- -CG-CUGG-AGCUUGUCCG--CGaCGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 6342 | 0.66 | 0.53282 |
Target: 5'- aGCGGCCUCGAGCA--CGUUGgUGUCGa -3' miRNA: 3'- -CGCUGGAGCUUGUccGCGAC-GCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 15703 | 0.66 | 0.531783 |
Target: 5'- cGCGGCCUCGAuacgcuuCAcGCGCUcaccgacGCGCUg- -3' miRNA: 3'- -CGCUGGAGCUu------GUcCGCGA-------CGCGGua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 31892 | 0.66 | 0.522482 |
Target: 5'- aGCGGCUUCGGcCA-GCGCgGCGCgGUu -3' miRNA: 3'- -CGCUGGAGCUuGUcCGCGaCGCGgUA- -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 64497 | 0.66 | 0.522482 |
Target: 5'- gGCGGCCU--GGCAGGCGggGUGCUu- -3' miRNA: 3'- -CGCUGGAgcUUGUCCGCgaCGCGGua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 33552 | 0.67 | 0.491961 |
Target: 5'- aGCGGCCgacaGcAGCAGGC-CaGCGCCGa -3' miRNA: 3'- -CGCUGGag--C-UUGUCCGcGaCGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 7221 | 0.67 | 0.491961 |
Target: 5'- aGCGAgCCgcugccGCGGGC-CUGCGCCAUc -3' miRNA: 3'- -CGCU-GGagcu--UGUCCGcGACGCGGUA- -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 59371 | 0.67 | 0.46913 |
Target: 5'- aGCGAUCgcgCGAGCcaacgauccauggaGGGCGCggcaGCGUCAg -3' miRNA: 3'- -CGCUGGa--GCUUG--------------UCCGCGa---CGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 15104 | 0.67 | 0.46913 |
Target: 5'- uGCGgcuucgccagguucGCCUCGAACuGGCGC-GCGUUg- -3' miRNA: 3'- -CGC--------------UGGAGCUUGuCCGCGaCGCGGua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 7865 | 0.68 | 0.427969 |
Target: 5'- cGCuuCCUCGGACAgGGUGCccuggcucacggugGCGCCAg -3' miRNA: 3'- -CGcuGGAGCUUGU-CCGCGa-------------CGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 33498 | 0.68 | 0.415035 |
Target: 5'- gGCGuCCUUGGGCAGcGCGaacaGCGCCu- -3' miRNA: 3'- -CGCuGGAGCUUGUC-CGCga--CGCGGua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 46718 | 0.69 | 0.39698 |
Target: 5'- cUGAUCUgGAGCAGGCGaaGCGCa-- -3' miRNA: 3'- cGCUGGAgCUUGUCCGCgaCGCGgua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 50155 | 0.69 | 0.379438 |
Target: 5'- uCGcCCUgGAGCAGGCGUacUGCGCg-- -3' miRNA: 3'- cGCuGGAgCUUGUCCGCG--ACGCGgua -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 18302 | 0.69 | 0.374278 |
Target: 5'- cUGGCCUCGccCGGGCGCagcacuggaacuugGCGCCGc -3' miRNA: 3'- cGCUGGAGCuuGUCCGCGa-------------CGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 60256 | 0.7 | 0.354117 |
Target: 5'- cGCGGCCgCGcGCGGGCuuucccGCcGCGCCAc -3' miRNA: 3'- -CGCUGGaGCuUGUCCG------CGaCGCGGUa -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 7309 | 0.7 | 0.337911 |
Target: 5'- -gGGCCagguccgguUCGAACugauaGGaGCGCUGCGCCAUc -3' miRNA: 3'- cgCUGG---------AGCUUG-----UC-CGCGACGCGGUA- -5' |
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30785 | 3' | -58.7 | NC_006552.1 | + | 11882 | 0.7 | 0.314626 |
Target: 5'- gGCGACCUUcucCAGGCGC-GCgGCCAa -3' miRNA: 3'- -CGCUGGAGcuuGUCCGCGaCG-CGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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