Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30787 | 3' | -50.7 | NC_006552.1 | + | 33857 | 0.66 | 0.939438 |
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Target: 5'- cGCCgAGCGGgAUGGuCUUGUaCUGGGc -3' miRNA: 3'- -CGG-UUGCCgUACUuGAACAaGGCCUc -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 16692 | 0.66 | 0.939438 |
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Target: 5'- gGCCAccGCGGCucccGAC-UGcgCCGGAGu -3' miRNA: 3'- -CGGU--UGCCGuac-UUGaACaaGGCCUC- -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 27750 | 0.67 | 0.928511 |
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Target: 5'- gGUguGCGGCGUGAgacgcgGCUUGgauggCUGGAa -3' miRNA: 3'- -CGguUGCCGUACU------UGAACaa---GGCCUc -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 10792 | 0.68 | 0.873463 |
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Target: 5'- cGCCGACGGCAUu-GCg---UCCGGcGa -3' miRNA: 3'- -CGGUUGCCGUAcuUGaacaAGGCCuC- -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 2922 | 0.73 | 0.61889 |
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Target: 5'- cGCCGGCGGCAUGGucgauauucACga---CCGGAGa -3' miRNA: 3'- -CGGUUGCCGUACU---------UGaacaaGGCCUC- -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 8931 | 0.79 | 0.33689 |
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Target: 5'- uGCCAACGGCGgacuuGACcUGUUCCGGGc -3' miRNA: 3'- -CGGUUGCCGUac---UUGaACAAGGCCUc -5' |
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| 30787 | 3' | -50.7 | NC_006552.1 | + | 19119 | 1.13 | 0.002039 |
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Target: 5'- cGCCAACGGCAUGAACUUGUUCCGGAGg -3' miRNA: 3'- -CGGUUGCCGUACUUGAACAAGGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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