Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30787 | 5' | -52.9 | NC_006552.1 | + | 21361 | 0.66 | 0.842255 |
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Target: 5'- -gCGCCGauucgaCGCUCAGGUcauugauaUAGCCGUg- -3' miRNA: 3'- gaGCGGCa-----GCGAGUUCA--------AUUGGCGau -5' |
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| 30787 | 5' | -52.9 | NC_006552.1 | + | 19798 | 0.67 | 0.785553 |
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Target: 5'- -cUGCaCcUCGCUCAGGUUGGCCuGCa- -3' miRNA: 3'- gaGCG-GcAGCGAGUUCAAUUGG-CGau -5' |
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| 30787 | 5' | -52.9 | NC_006552.1 | + | 35089 | 0.68 | 0.744338 |
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Target: 5'- uCUCGgCGUCGCU--GGU--GCCGCUc -3' miRNA: 3'- -GAGCgGCAGCGAguUCAauUGGCGAu -5' |
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| 30787 | 5' | -52.9 | NC_006552.1 | + | 63088 | 0.69 | 0.668181 |
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Target: 5'- -aCGCCGUCGC-CGAGaacuGCCGUg- -3' miRNA: 3'- gaGCGGCAGCGaGUUCaau-UGGCGau -5' |
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| 30787 | 5' | -52.9 | NC_006552.1 | + | 32890 | 0.71 | 0.575906 |
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Target: 5'- -aCGCCGUCGCUCGccaucugcgccaccAGg--GCCGCa- -3' miRNA: 3'- gaGCGGCAGCGAGU--------------UCaauUGGCGau -5' |
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| 30787 | 5' | -52.9 | NC_006552.1 | + | 19155 | 1.07 | 0.002567 |
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Target: 5'- aCUCGCCGUCGCUCAAGUUAACCGCUAc -3' miRNA: 3'- -GAGCGGCAGCGAGUUCAAUUGGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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