Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30788 | 5' | -58 | NC_006552.1 | + | 30906 | 0.66 | 0.598309 |
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Target: 5'- gCUGgcaGCGGUcGgGCGGacauGGAGCCUGCUg -3' miRNA: 3'- -GAC---UGCCA-CgUGUCgc--UCUCGGACGA- -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 49265 | 0.66 | 0.587557 |
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Target: 5'- aCUGgcACGG-GCuGCGGCGcuuacguGGGCCUGCa -3' miRNA: 3'- -GAC--UGCCaCG-UGUCGCu------CUCGGACGa -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 12963 | 0.66 | 0.587557 |
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Target: 5'- cCUGGCGGUGCGuu-CGAG-GCCaGCa -3' miRNA: 3'- -GACUGCCACGUgucGCUCuCGGaCGa -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 63625 | 0.67 | 0.517855 |
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Target: 5'- uUGACGGaccuCGacgacguucacucuuCAGcCGAGAGCCUGCUg -3' miRNA: 3'- gACUGCCac--GU---------------GUC-GCUCUCGGACGA- -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 27746 | 0.68 | 0.483222 |
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Target: 5'- -aGAUGGUGUGCGGCGuGAGaCgcgGCUu -3' miRNA: 3'- gaCUGCCACGUGUCGCuCUCgGa--CGA- -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 51982 | 0.71 | 0.32224 |
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Target: 5'- aCUGGCac-GCcaGCAGCGAGAGCUUGCc -3' miRNA: 3'- -GACUGccaCG--UGUCGCUCUCGGACGa -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 7212 | 0.71 | 0.32224 |
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Target: 5'- ---cCGGcGCGCAGCGAGccgcugccgcgGGCCUGCg -3' miRNA: 3'- gacuGCCaCGUGUCGCUC-----------UCGGACGa -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 12801 | 0.75 | 0.179517 |
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Target: 5'- cCUGGCGGaaCuCGGCGAGGGUCUGCg -3' miRNA: 3'- -GACUGCCacGuGUCGCUCUCGGACGa -5' |
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| 30788 | 5' | -58 | NC_006552.1 | + | 18877 | 1.08 | 0.000701 |
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Target: 5'- gCUGACGGUGCACAGCGAGAGCCUGCUg -3' miRNA: 3'- -GACUGCCACGUGUCGCUCUCGGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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