miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
30792 3' -56.8 NC_006552.1 + 15118 1.03 0.001746
Target:  5'- gUUCGCCUCGAACUGGCGCGCGUUGAAc -3'
miRNA:   3'- -AAGCGGAGCUUGACCGCGCGCAACUU- -5'
30792 3' -56.8 NC_006552.1 + 13219 0.69 0.438469
Target:  5'- gUCGCCUCcccACUGcGCGCGCGcugcaUGAGg -3'
miRNA:   3'- aAGCGGAGcu-UGAC-CGCGCGCa----ACUU- -5'
30792 3' -56.8 NC_006552.1 + 20321 0.68 0.509052
Target:  5'- -gCGCgUUGAACaGGCugaucuGCGCGUUGAAc -3'
miRNA:   3'- aaGCGgAGCUUGaCCG------CGCGCAACUU- -5'
30792 3' -56.8 NC_006552.1 + 33521 0.67 0.562253
Target:  5'- -gCGCCUUgcugGAACUGGUGCGCa----- -3'
miRNA:   3'- aaGCGGAG----CUUGACCGCGCGcaacuu -5'
30792 3' -56.8 NC_006552.1 + 12953 0.67 0.573091
Target:  5'- cUgGCCUUGGcCUGGCGgUGCGUUcGAGg -3'
miRNA:   3'- aAgCGGAGCUuGACCGC-GCGCAA-CUU- -5'
30792 3' -56.8 NC_006552.1 + 32479 0.67 0.573091
Target:  5'- -gCGCCacCGAACUGGUcgauGCGCGcgUGGAc -3'
miRNA:   3'- aaGCGGa-GCUUGACCG----CGCGCa-ACUU- -5'
30792 3' -56.8 NC_006552.1 + 56921 0.66 0.627834
Target:  5'- -gCGCCcgcgcUGGACUGGCGCaacuaccccgauGCGUUGGc -3'
miRNA:   3'- aaGCGGa----GCUUGACCGCG------------CGCAACUu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.