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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30793 | 3' | -58.4 | NC_006552.1 | + | 15635 | 0.66 | 0.643487 |
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Target: 5'- gCGUUcUgGUGGcGCUGCuCAUCCuGCUGGu -3' miRNA: 3'- -GCAAcAgCGCC-CGGCG-GUAGG-UGACC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 19736 | 0.66 | 0.622391 |
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Target: 5'- aCGUUGgCGCuGGCCGCUGcgcgccaggcuUCUAcCUGGg -3' miRNA: 3'- -GCAACaGCGcCCGGCGGU-----------AGGU-GACC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 41005 | 0.67 | 0.580356 |
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Target: 5'- uGUUGUUGUGGGCgaacaGCaguuCAUCCGCguUGGc -3' miRNA: 3'- gCAACAGCGCCCGg----CG----GUAGGUG--ACC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 24912 | 0.68 | 0.477946 |
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Target: 5'- --aUGUCGauGGCCaccgccuGCCGaCCGCUGGg -3' miRNA: 3'- gcaACAGCgcCCGG-------CGGUaGGUGACC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 21212 | 0.7 | 0.386964 |
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Target: 5'- uGUUGcCGauGGCCGCCAgagcUUCGCUGa -3' miRNA: 3'- gCAACaGCgcCCGGCGGU----AGGUGACc -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 12226 | 0.73 | 0.253251 |
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Target: 5'- ---aG-CGCGGGCgGCCAUCCGCgcacGGc -3' miRNA: 3'- gcaaCaGCGCCCGgCGGUAGGUGa---CC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 7227 | 0.75 | 0.20196 |
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Target: 5'- cCGcUGcCGCGGGCCugcGCCAUCaACUGGu -3' miRNA: 3'- -GCaACaGCGCCCGG---CGGUAGgUGACC- -5' |
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| 30793 | 3' | -58.4 | NC_006552.1 | + | 14529 | 1.1 | 0.000631 |
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Target: 5'- cCGUUGUCGCGGGCCGCCAUCCACUGGu -3' miRNA: 3'- -GCAACAGCGCCCGGCGGUAGGUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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