Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30796 | 3' | -62.2 | NC_006552.1 | + | 15215 | 0.66 | 0.394328 |
Target: 5'- cCGGUCGCCUUGCGguuGGUGUccacGCUGa -3' miRNA: 3'- aGCCGGCGGAGCGC---CCACGccu-UGAC- -5' |
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30796 | 3' | -62.2 | NC_006552.1 | + | 41426 | 0.67 | 0.384983 |
Target: 5'- uUCGGCCGCgUcCGUGGGgaGCGGcaucaguGGCUc -3' miRNA: 3'- -AGCCGGCGgA-GCGCCCa-CGCC-------UUGAc -5' |
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30796 | 3' | -62.2 | NC_006552.1 | + | 58937 | 0.7 | 0.241058 |
Target: 5'- aCGGCCaGCCUUGCcacguugcgGCGGAACUGu -3' miRNA: 3'- aGCCGG-CGGAGCGccca-----CGCCUUGAC- -5' |
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30796 | 3' | -62.2 | NC_006552.1 | + | 32704 | 0.7 | 0.230629 |
Target: 5'- cCGGCCGg--CGCGGGgccgGUGGAGCg- -3' miRNA: 3'- aGCCGGCggaGCGCCCa---CGCCUUGac -5' |
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30796 | 3' | -62.2 | NC_006552.1 | + | 12433 | 1.09 | 0.000311 |
Target: 5'- uUCGGCCGCCUCGCGGGUGCGGAACUGg -3' miRNA: 3'- -AGCCGGCGGAGCGCCCACGCCUUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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