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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30798 | 3' | -51.2 | NC_006552.1 | + | 30087 | 0.66 | 0.941002 |
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Target: 5'- cGGGUAGCagGCUGgccagccuGGUCCAc-GACGGu- -3' miRNA: 3'- -CCCAUCG--CGAC--------CCAGGUauUUGCUug -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 42206 | 0.66 | 0.918388 |
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Target: 5'- uGGGUcGCGUUcaGGUCUAccagguauuccUGGACGAACg -3' miRNA: 3'- -CCCAuCGCGAc-CCAGGU-----------AUUUGCUUG- -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 41051 | 0.68 | 0.850351 |
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Target: 5'- aGGGaAGCGCUcgcggauuuccucGGGcagguugUCGUAGGCGAACu -3' miRNA: 3'- -CCCaUCGCGA-------------CCCa------GGUAUUUGCUUG- -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 33180 | 0.69 | 0.824297 |
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Target: 5'- gGGGUGGUGuCUGGGuugUCCA-GGugGAGu -3' miRNA: 3'- -CCCAUCGC-GACCC---AGGUaUUugCUUg -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 25299 | 0.69 | 0.823366 |
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Target: 5'- cGGGUAGCGgUgucggucGGcGUCCAagcUGGGCGggUa -3' miRNA: 3'- -CCCAUCGCgA-------CC-CAGGU---AUUUGCuuG- -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 15641 | 0.7 | 0.733455 |
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Target: 5'- uGGUGGCGCUGcucauccugcuGGUCCuc-GGCGAGg -3' miRNA: 3'- cCCAUCGCGAC-----------CCAGGuauUUGCUUg -5' |
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| 30798 | 3' | -51.2 | NC_006552.1 | + | 10918 | 1.12 | 0.002017 |
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Target: 5'- cGGGUAGCGCUGGGUCCAUAAACGAACc -3' miRNA: 3'- -CCCAUCGCGACCCAGGUAUUUGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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