Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30798 | 5' | -59 | NC_006552.1 | + | 14481 | 0.66 | 0.5501 |
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Target: 5'- aCCaggUCGCggaugGG-GGCAUCCAGGCgGa -3' miRNA: 3'- -GGca-AGUGa----CCaCCGUGGGUCCGgCa -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 18140 | 0.67 | 0.519375 |
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Target: 5'- cCCGUUaAC-GGUGuaGCCCGGGCgGa -3' miRNA: 3'- -GGCAAgUGaCCACcgUGGGUCCGgCa -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 38454 | 0.67 | 0.499271 |
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Target: 5'- gCUGUUCcuggaugccGCUGGUGGCcgugccuuugcGCCCcuggaaaccGGCCGUg -3' miRNA: 3'- -GGCAAG---------UGACCACCG-----------UGGGu--------CCGGCA- -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 12179 | 0.7 | 0.370181 |
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Target: 5'- uUGUUCA-UGGUGGCGgCCAGuGCCu- -3' miRNA: 3'- gGCAAGUgACCACCGUgGGUC-CGGca -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 61718 | 0.7 | 0.345631 |
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Target: 5'- aCCGcgCGCgGGUacGCGCCCuGGCCGa -3' miRNA: 3'- -GGCaaGUGaCCAc-CGUGGGuCCGGCa -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 63802 | 0.71 | 0.322271 |
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Target: 5'- aCCGgugCGCUaGcuucGGCGCCCAGGCCa- -3' miRNA: 3'- -GGCaa-GUGAcCa---CCGUGGGUCCGGca -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 61492 | 0.71 | 0.314751 |
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Target: 5'- gCCGgUCgACUacGUGcGCGCCCGGGCCGa -3' miRNA: 3'- -GGCaAG-UGAc-CAC-CGUGGGUCCGGCa -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 61433 | 0.73 | 0.212196 |
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Target: 5'- aCGUUCACcc---GCGCCCGGGCCGUa -3' miRNA: 3'- gGCAAGUGaccacCGUGGGUCCGGCA- -5' |
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| 30798 | 5' | -59 | NC_006552.1 | + | 10954 | 1.03 | 0.001689 |
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Target: 5'- gCCGUUCACUGGUGGCACCCAGGCCu- -3' miRNA: 3'- -GGCAAGUGACCACCGUGGGUCCGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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