Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30800 | 5' | -55.3 | NC_006552.1 | + | 28812 | 0.66 | 0.697466 |
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Target: 5'- -gACCAGGgUgGCGGGuucaacucGCUGUccGGGUCg -3' miRNA: 3'- aaUGGUUCgA-CGCCCu-------UGACG--UCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 17444 | 0.66 | 0.675394 |
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Target: 5'- ----gAAGUUGCauGGACUGCGGGUCg -3' miRNA: 3'- aauggUUCGACGccCUUGACGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 25493 | 0.66 | 0.664289 |
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Target: 5'- -cGCCAGGCccucgGUGGGAAU--CAGGUUg -3' miRNA: 3'- aaUGGUUCGa----CGCCCUUGacGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 12798 | 0.67 | 0.619667 |
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Target: 5'- cUGCCuGGCggaacucgGCGaGGGuCUGCGGGUUg -3' miRNA: 3'- aAUGGuUCGa-------CGC-CCUuGACGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 47661 | 0.68 | 0.586281 |
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Target: 5'- -gGCC-GGCUGuCGGGAuCU-CAGGUCc -3' miRNA: 3'- aaUGGuUCGAC-GCCCUuGAcGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 10501 | 0.68 | 0.553248 |
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Target: 5'- cUGCCAc----CGGGAuacGCUGCAGGUCa -3' miRNA: 3'- aAUGGUucgacGCCCU---UGACGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 21483 | 0.73 | 0.314263 |
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Target: 5'- ---gCAGGCUcgcucuccaGCGGGuAGCUGUAGGUCg -3' miRNA: 3'- aaugGUUCGA---------CGCCC-UUGACGUCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 58946 | 0.74 | 0.249547 |
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Target: 5'- cUUGCCAcGUUGCGgcGGAACUGUguAGGUCg -3' miRNA: 3'- -AAUGGUuCGACGC--CCUUGACG--UCCAG- -5' |
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| 30800 | 5' | -55.3 | NC_006552.1 | + | 10046 | 1.08 | 0.001077 |
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Target: 5'- cUUACCAAGCUGCGGGAACUGCAGGUCg -3' miRNA: 3'- -AAUGGUUCGACGCCCUUGACGUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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