Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30802 | 5' | -54.4 | NC_006552.1 | + | 25469 | 0.66 | 0.78725 |
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Target: 5'- gCCCAGCGCGaugUUgaggaugUGCgccaGGCCcucggUGGGa -3' miRNA: 3'- gGGGUCGCGU---AAa------ACGa---CCGGa----ACCU- -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 48317 | 0.66 | 0.777285 |
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Target: 5'- aCCCGGCgGCGaccgUUGCUG-CCU-GGAa -3' miRNA: 3'- gGGGUCG-CGUaa--AACGACcGGAaCCU- -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 7209 | 0.67 | 0.749655 |
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Target: 5'- uCCCCGGCGCGcagcgagccgcUGCcgcgGGCCUgcgccaucaacuggUGGAc -3' miRNA: 3'- -GGGGUCGCGUaaa--------ACGa---CCGGA--------------ACCU- -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 18832 | 0.67 | 0.746527 |
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Target: 5'- aUCCCGGCGCAacac-CUGGCU--GGAg -3' miRNA: 3'- -GGGGUCGCGUaaaacGACCGGaaCCU- -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 38141 | 0.68 | 0.671209 |
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Target: 5'- gCCUCAGacauCGCGUUgagcUGCUGGCCgcGGu -3' miRNA: 3'- -GGGGUC----GCGUAAa---ACGACCGGaaCCu -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 12636 | 0.69 | 0.594034 |
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Target: 5'- gCCCGGCGCuggcuacGCgGGUCUUGGc -3' miRNA: 3'- gGGGUCGCGuaaaa--CGaCCGGAACCu -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 60274 | 0.7 | 0.561254 |
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Target: 5'- uUCCCGccGCGCcacccgGCUGGCCcUGGGc -3' miRNA: 3'- -GGGGU--CGCGuaaaa-CGACCGGaACCU- -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 38731 | 0.7 | 0.550431 |
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Target: 5'- gCCCCAGCGCAaUUUGCUGacaCCa---- -3' miRNA: 3'- -GGGGUCGCGUaAAACGACc--GGaaccu -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 12944 | 0.74 | 0.322207 |
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Target: 5'- -gCCAGCGC-----GCUGGCCUUGGc -3' miRNA: 3'- ggGGUCGCGuaaaaCGACCGGAACCu -5' |
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| 30802 | 5' | -54.4 | NC_006552.1 | + | 6978 | 0.99 | 0.007363 |
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Target: 5'- cCCCCAGCG-AUUUUGCUGGCCUUGGAg -3' miRNA: 3'- -GGGGUCGCgUAAAACGACCGGAACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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