Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30804 | 3' | -58.1 | NC_006552.1 | + | 6440 | 1.08 | 0.000729 |
Target: 5'- cCGCGCCCGGCGACAUCUCCAGGAUACc -3' miRNA: 3'- -GCGCGGGCCGCUGUAGAGGUCCUAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 56819 | 0.73 | 0.237717 |
Target: 5'- gCGCGCCCGGCGgaugguuguccGCAgcuUCCAGGGa-- -3' miRNA: 3'- -GCGCGGGCCGC-----------UGUag-AGGUCCUaug -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 61507 | 0.73 | 0.237717 |
Target: 5'- gCGCGCCCGGgccgaguaCGGCGUCgaucaCCAGGAc-- -3' miRNA: 3'- -GCGCGGGCC--------GCUGUAGa----GGUCCUaug -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 31986 | 0.7 | 0.367217 |
Target: 5'- gGUGCUCGGCGugAaUCUCC-GGcUGCg -3' miRNA: 3'- gCGCGGGCCGCugU-AGAGGuCCuAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 1918 | 0.69 | 0.429189 |
Target: 5'- aCGUGCUcgaCGGCGGCGUCguugUCGGGAg-- -3' miRNA: 3'- -GCGCGG---GCCGCUGUAGa---GGUCCUaug -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 52951 | 0.69 | 0.429189 |
Target: 5'- gGCGCCCGccgccgguGCGGCA--UCCGGG-UGCg -3' miRNA: 3'- gCGCGGGC--------CGCUGUagAGGUCCuAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 12878 | 0.69 | 0.45757 |
Target: 5'- uGCGCCuCGGCGACcUCggCGGcGUGCg -3' miRNA: 3'- gCGCGG-GCCGCUGuAGagGUCcUAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 32967 | 0.68 | 0.50597 |
Target: 5'- aCGCGCgCGuCGGCGUCcuggcucccagcgUCCGGGAUGu -3' miRNA: 3'- -GCGCGgGCcGCUGUAG-------------AGGUCCUAUg -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 15797 | 0.68 | 0.506981 |
Target: 5'- uCGCGCUCGGCGauuuucuucGCGUCccaUCCAGuGGcACg -3' miRNA: 3'- -GCGCGGGCCGC---------UGUAG---AGGUC-CUaUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 25896 | 0.68 | 0.506981 |
Target: 5'- gGCGaCCGGCGAgccaUCCGGuGAUGCg -3' miRNA: 3'- gCGCgGGCCGCUguagAGGUC-CUAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 60835 | 0.67 | 0.558525 |
Target: 5'- gGCgaGCCCGGCGAUcggUUguGGGUGCg -3' miRNA: 3'- gCG--CGGGCCGCUGuagAGguCCUAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 39604 | 0.67 | 0.569026 |
Target: 5'- aGCGCCgGGcCGAUcagGUCUUCcacugcgggcGGGAUGCc -3' miRNA: 3'- gCGCGGgCC-GCUG---UAGAGG----------UCCUAUG- -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 54257 | 0.66 | 0.579576 |
Target: 5'- gCGCGCCCuGCG-CcUC-CCAGGAg-- -3' miRNA: 3'- -GCGCGGGcCGCuGuAGaGGUCCUaug -5' |
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30804 | 3' | -58.1 | NC_006552.1 | + | 26513 | 0.66 | 0.632778 |
Target: 5'- gGCGCCUGccaccagcaGCGGCGgcgCCGGGGUGu -3' miRNA: 3'- gCGCGGGC---------CGCUGUagaGGUCCUAUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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