miRNA: 3'- cuCGCGA---UGUUGCa--ACGCGgUAGCUG- -5'
miRNA: 3'- -CUC-GCGAUGuUGCa---ACGCGGUAGcUG- -5'
miRNA: 3'- cUCGCGA--------UGUUGCaaCGCGGUAGCUG- -5'
miRNA: 3'- -CUCGCGAUGUUGCAACGCGGUAGCUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30812 | 3' | -53.2 | NC_006552.1 | + | 12374 | 0.72 | 0.494953 |
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Target: 5'- -cGCGCUucuGCAACGgccUGCGC-GUCGGCg -3' miRNA: 3'- cuCGCGA---UGUUGCa--ACGCGgUAGCUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 7223 | 0.74 | 0.378081 |
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Target: 5'- cGAGcCGCUGCcGCGggccUGCGCCAUCaACu -3' miRNA: 3'- -CUC-GCGAUGuUGCa---ACGCGGUAGcUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 25204 | 0.78 | 0.240691 |
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Target: 5'- uGGUGCUguuggucaGCAGCGcgGCGCCGUUGACg -3' miRNA: 3'- cUCGCGA--------UGUUGCaaCGCGGUAGCUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 2556 | 1.11 | 0.001373 |
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Target: 5'- aGAGCGCUACAACGUUGCGCCAUCGACg -3' miRNA: 3'- -CUCGCGAUGUUGCAACGCGGUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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