miRNA: 3'- aGUUAGCGaAC-GGCG-CUGUCGCCGUg -5'
miRNA: 3'- -AGUUAGCGaa--CGGC-------GCUGUCGCCGUG- -5'
miRNA: 3'- agUUAG-CGaACGGCGC-UGUCGCCGug -5'
miRNA: 3'- -AGUUAGCGAACGGCGCUGUCGCCGUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30814 | 5' | -56 | NC_006552.1 | + | 22001 | 0.72 | 0.37005 |
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Target: 5'- cCAGUCGCcUGaUCGCuGACGGUGGCGg -3' miRNA: 3'- aGUUAGCGaAC-GGCG-CUGUCGCCGUg -5' |
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| 30814 | 5' | -56 | NC_006552.1 | + | 24257 | 0.72 | 0.335748 |
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Target: 5'- aUCGGUUGUggucGCCGgcacuacCGGCAGCGGCAUa -3' miRNA: 3'- -AGUUAGCGaa--CGGC-------GCUGUCGCCGUG- -5' |
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| 30814 | 5' | -56 | NC_006552.1 | + | 32337 | 0.74 | 0.262735 |
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Target: 5'- -gGAUCaGCcUGCCGCGcACGGCGGCu- -3' miRNA: 3'- agUUAG-CGaACGGCGC-UGUCGCCGug -5' |
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| 30814 | 5' | -56 | NC_006552.1 | + | 340 | 1.1 | 0.00073 |
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Target: 5'- cUCAAUCGCUUGCCGCGACAGCGGCACg -3' miRNA: 3'- -AGUUAGCGAACGGCGCUGUCGCCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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