miRNA: 3'- gACGACCAGgC-------AGGAGUuaCUUCGAG- -5'
miRNA: 3'- gACGACCag-GCAGGAGUUacUUCGag -5'
miRNA: 3'- gaCGACCAGGCaggAGUUACUu-----CGAG- -5'
miRNA: 3'- -GACGACCaGGCaGGAGUUaC-UUCGAg -5'
miRNA: 3'- -GACGAC-CAGGcaGGAGUUACU-------UCGAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30816 | 5' | -53.3 | NC_006552.1 | + | 13486 | 0.68 | 0.711074 |
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Target: 5'- aUGCUGGUCgGguagccaUCCUUGccGAAGUUCa -3' miRNA: 3'- gACGACCAGgC-------AGGAGUuaCUUCGAG- -5' |
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| 30816 | 5' | -53.3 | NC_006552.1 | + | 38183 | 0.68 | 0.701258 |
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Target: 5'- gUGCUGGgaaCGUCCUCGA--GGGCg- -3' miRNA: 3'- gACGACCag-GCAGGAGUUacUUCGag -5' |
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| 30816 | 5' | -53.3 | NC_006552.1 | + | 40597 | 0.69 | 0.645937 |
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Target: 5'- -cGCUGGUCCGgg-UCGAUGGugaugcGCUCg -3' miRNA: 3'- gaCGACCAGGCaggAGUUACUu-----CGAG- -5' |
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| 30816 | 5' | -53.3 | NC_006552.1 | + | 55228 | 0.71 | 0.535504 |
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Target: 5'- cCUGCUGG-CCGaCCUCAAcGaAAGCUa -3' miRNA: 3'- -GACGACCaGGCaGGAGUUaC-UUCGAg -5' |
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| 30816 | 5' | -53.3 | NC_006552.1 | + | 2302 | 0.74 | 0.366605 |
|
Target: 5'- cCUGCUGuGUCC--CCUCGAUGAauucagcAGCUCa -3' miRNA: 3'- -GACGAC-CAGGcaGGAGUUACU-------UCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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