Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30817 | 3' | -57.4 | NC_006552.1 | + | 8419 | 0.66 | 0.676727 |
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Target: 5'- gGGGCUCAGgCCGCGccaGgCGCGCa-- -3' miRNA: 3'- gUCUGAGUUgGGUGCa--CgGCGCGagc -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 4627 | 0.66 | 0.6447 |
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Target: 5'- gGGAaUCAACCgggcgcgaCACGaauuguagGCCGCGCUCa -3' miRNA: 3'- gUCUgAGUUGG--------GUGCa-------CGGCGCGAGc -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 36386 | 0.67 | 0.612574 |
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Target: 5'- gCGGAUUac-UCCGCGUcucGCCGgCGCUCGg -3' miRNA: 3'- -GUCUGAguuGGGUGCA---CGGC-GCGAGC- -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 18516 | 0.67 | 0.601886 |
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Target: 5'- gCAuACUCAcccgauGCCCAC-UGCUGCGCUaCGu -3' miRNA: 3'- -GUcUGAGU------UGGGUGcACGGCGCGA-GC- -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 13392 | 0.67 | 0.601886 |
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Target: 5'- -cGACUcCGGCaCGCucaUGCCGCGCUCa -3' miRNA: 3'- guCUGA-GUUGgGUGc--ACGGCGCGAGc -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 49859 | 0.67 | 0.601886 |
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Target: 5'- -cGGCUCGACgCCGCGUcggacGCC-CGCUgGg -3' miRNA: 3'- guCUGAGUUG-GGUGCA-----CGGcGCGAgC- -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 29011 | 0.67 | 0.591222 |
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Target: 5'- -cGACUCGggaaugAUCCGCGUGCCGaauGC-CGa -3' miRNA: 3'- guCUGAGU------UGGGUGCACGGCg--CGaGC- -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 47836 | 0.68 | 0.548971 |
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Target: 5'- -uGAC-CAACCCGCGUGgCGuCGCg-- -3' miRNA: 3'- guCUGaGUUGGGUGCACgGC-GCGagc -5' |
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| 30817 | 3' | -57.4 | NC_006552.1 | + | 62624 | 0.68 | 0.517906 |
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Target: 5'- -uGAgUCuGCCCGCaUGCCaGCGUUCGa -3' miRNA: 3'- guCUgAGuUGGGUGcACGG-CGCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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