Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30819 | 3' | -61.9 | NC_006552.1 | + | 21178 | 0.68 | 0.260488 |
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Target: 5'- -gGCC-GCGUCGGUGC-CgGCACAUc -3' miRNA: 3'- uaCGGuCGCGGCCACGcGgCGUGUAc -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 7716 | 0.69 | 0.254113 |
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Target: 5'- -aGCCGGCGCau-UGCGCUGCGguUGg -3' miRNA: 3'- uaCGGUCGCGgccACGCGGCGUguAC- -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 7620 | 0.7 | 0.207643 |
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Target: 5'- -aGaCCGGCGgCGGUuuGCCGCGCAUu -3' miRNA: 3'- uaC-GGUCGCgGCCAcgCGGCGUGUAc -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 41147 | 0.72 | 0.151786 |
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Target: 5'- gAUGCCgcAGCGCUGGU-CGCCGUugaacagcGCAUGg -3' miRNA: 3'- -UACGG--UCGCGGCCAcGCGGCG--------UGUAC- -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 58610 | 0.73 | 0.122507 |
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Target: 5'- -gGUgGGCGUCGGUG-GCCGCGCcgGg -3' miRNA: 3'- uaCGgUCGCGGCCACgCGGCGUGuaC- -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 51893 | 0.75 | 0.093285 |
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Target: 5'- -aGCCAGCaaGCCGaaaUGCGCCGcCGCAUGg -3' miRNA: 3'- uaCGGUCG--CGGCc--ACGCGGC-GUGUAC- -5' |
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| 30819 | 3' | -61.9 | NC_006552.1 | + | 48877 | 0.76 | 0.074795 |
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Target: 5'- -gGCCA-CGCCGGUGCuGCUGCGCAa- -3' miRNA: 3'- uaCGGUcGCGGCCACG-CGGCGUGUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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